水产学报2018，Vol.42Issue(4)：495-502,8.DOI:10.11964/jfc.20170310744
枯草芽孢杆菌肽聚糖对β-伴大豆球蛋白诱导的鲤幼鱼肠上皮细胞损伤的保护作用
Protective effect of Bacillus subtilis peptidoglycan on β-conglycinin-induced intestinal epithelial cells damage of juvenile carp(Cyprinus carpio)
摘要
Abstract
This experiment was conducted to investigate the protective effects of different concentration of Bacil-lus subtilis peptidoglycan on the β-conglycinin-induced inflammatory lesions in intestinal epithelial cells of juven-ile carp(Cyprinus carpio).In 24-cell microplates,the intestinal epithelial cells(IECs)of juvenile carp were primarily cultured for 72 h at 26 °C and 6% CO2, and then the IECs were randomly divided into 6 groups with 4 replicates per group. One of the 6 groups was set as negative control group, and the other groups were all supple-mented with 1.0 mg/mL β-conglycinin in culture medium in order to establish inflammatory lesions. After 24 h further culture in the same conditions,the culture medium was changed into the B.subtilis peptidoglycan culture medium with 0 (positive control group), 0.15, 0.30, 0.45 and 0.60 mg/mL, respectively. The samples were collec-ted to measure the antioxidant and anti-inflammatory indices after 12,24 and 36 h B.subtilis peptidoglycan cul-ture. The results showed as follows: β-conglycinin exposure significantly decreased the activity of ASA, AHR, SOD, CAT, GPx, and increased the content of PC and the mRNA expression of inflammatory cytokines (IL-1β, IL-8, TNF-α1, IL-10 and TGF-β). The 12, 24 and 36 hours after the peptidoglycan were given, the activity of ASA, AHR, SOD, CAT, GPx and the content of PC in the cells were decreased in a dose-dependent manner; and the mRNA levels of IL-1β, IL-8 and TNF-α1 were down-regulated and IL-10 and TGF-β were up-regulated. Different concentration of B.subtilis peptidoglycan could significantly improve the anti-oxidative activity,and increase the mRNA levels of anti-inflammation cytokine, and decrease the pro-inflammation cytokine, and inhibit the inflam-matory response of the carp IECs induced by β-conglycinin.关键词
鲤/枯草芽孢杆菌肽聚糖/β-伴大豆球蛋白/肠上皮细胞/抗氧化/炎性因子Key words
Cyprinus carpio/Bacillus subtilis peptidoglycan/β-conglycinin/intestinal epithelial cell/antioxidant/inflammatory factor分类
农业科技引用本文复制引用
张甜甜,殷海成,黄巍..枯草芽孢杆菌肽聚糖对β-伴大豆球蛋白诱导的鲤幼鱼肠上皮细胞损伤的保护作用[J].水产学报,2018,42(4):495-502,8.基金项目
河南省科技攻关项目(172102110205)Science and Technology Research Project of Henan (172102110205) （172102110205）
