水产学报2018，Vol.42Issue(4)：534-543,10.DOI:10.11964/jfc.20170310767
尿囊素对印度产紫菜Pyropia chauhanii单孢子放散和体细胞分化的影响
The influence of allantoin on monospores release and somatic cell differentiation in gametophytic blades of Pyropia chauhanii
摘要
Abstract
In order to explore the influence of allantoin on monospores formation and release of Pyropia chauh-anii,we used the wild-type strain(PC-WT)which could release monospore and its induced strain(PC-Y1)which could not release monospore as materials, then different strains blade discs of apical, middle and basal sections were cultured in medium adjusted with different concentration of allantoin. The research results showed that, in 6 days after treatment, the order of the release number of the different parts of each disc was middle>apical>basal;With the increasing of allantoin concentration,the numbers of release monospores of PC-WT strain blade discs showed a trend of increasing first and then decreasing, and 10 mmol/L was the optimal allantoin concentration of promoting monospore release.But allantoin could not promote PC-Y1 release monospore.We used 20 mmol/L al-lantoin of medium to culture the discs of PC-Y 1 blade for 8 days,and single somatic cell was obtained using en-zymatic hydrolysis from the discs of different section,and continue culturing in vitro.We found that the percent-age of single cells developed into conchocelis was 66.1% in treatment group; and the percentage of single cells de-veloped into conchocelis was only 15.2% in control group. The result showed that allantoin could promote differ-entiation from somatic cell to germ cell of blade in Pyropia.关键词
Pyropiachauhanii/尿囊素/单孢子放散/体细胞分化/丝状体Key words
Pyropia chauhanii/allantoin/monospores release/somatic cell differentiation/conchocelis分类
农业科技引用本文复制引用
孙迪,丁洪昌,严兴洪..尿囊素对印度产紫菜Pyropia chauhanii单孢子放散和体细胞分化的影响[J].水产学报,2018,42(4):534-543,10.基金项目
国家"八六三"高技术研究发展计划(2012AA10A411) （2012AA10A411）
国家自然科学基金(31072208) （31072208）
浙江省农业(水产)新品种选育重大科技专项(2016C02055-6) （水产）
年产2000吨重要经济红藻规模化养殖及产业化(2014FJ19)National High Technology Research & Development Program of China (2012AA10A411) （2014FJ19）
Na-tional Natural Science Foundation of China (31072208) （31072208）
Major Science and Technology Special Fund of Agricul-ture (Fisheries) New Variety Breeding of Zhejiang Province, China (2016C02055-6) （Fisheries）
Large-scale Cultivation and Industrialization of Important Economic Red Algae with an Annual Output of 2000 Tons (2014FJ19) （2014FJ19）
