水产学报2018，Vol.42Issue(4)：557-564,8.DOI:10.11964/jfc.20170410813
白斑狗鱼雌性化诱导和雌雄同体现象
Feminization induction and hermaphroditism of northern pike(Esox lucius)
摘要
Abstract
Northern pike(Esox lucius)is a very popular edible fish with high quality and an economically import-ant fish. In China, northern pik is only distributed in the Ertixche River in northern Xinjiang. Northern pike fe-males grow faster than males. To investigate and develop the feminization induction method on northern pike, 750 fries of this fish were divided into five groups in this study. Among them, four groups were used as experimental groups,and fed with Tubifex hattai,which had been soaked with 20 mg/L 17β-estradiol,starting from different de-velopmental time (10 d, 20 d, 30 d and 40 d, respectively after hatching). The other one group was used as control group,and fed with normal T.hattai.All fish in experimental groups were fed with 17β-estradiol for 30 d,and then all the fish in the experimental groups and the control group were fed normally. On 206 d, survival situations of the fish in all groups were counted, and then all the fish were dissected, gonads were removed to perform histological observation.The results showed that feeding with T.hattai,which had been soaked with 20 mg/L 17β-estradiol, had very obvious lethal effect on northern pike fries, and made the survival rate decreased from 43.5% in the con-trol group to 14.3%±8.3%. Higher ratios of female to male were obtained in 30 d treated group and 40 d treated group, especially the highest ratio, which reached 15:7, was obtained in the 30 d treated group, so it can be con-cluded that the key time point of gonad differentiation of northern pike may be in the period of 30–40 d after hatching. There were high proportions of hermaphroditism in both experimental and control groups, that is, a large number of primary oocytes (phase II) and spermatocytes appeared simultaneously in the same gland, and moreover, these oocytes showed marked degeneration. It is presumed that there was a protogynous juvenile herm-aphroditism in the process of sexual differentiation of northern pike. This study will provide certain basis for pro-duction of all female northern pike fries using hormone induction techniques and further research on sex determin-ing mechanism of northern pike.关键词
白斑狗鱼/17β-雌二醇/雌性化诱导/雌雄同体Key words
Esox lucius/17β-estradiol/feminization induction/hermaphroditism分类
农业科技引用本文复制引用
张俊杰,胡琼,管潇萌,李飞,王明坤,李胜忠..白斑狗鱼雌性化诱导和雌雄同体现象[J].水产学报,2018,42(4):557-564,8.基金项目
国家自然科学基金(31660739) （31660739）
新疆维吾尔自治区高校科研计划项目(XJEDU2016I021) （XJEDU2016I021）
新疆农业大学大学生创新项目(201610758124)National Natural Science Foundation of China (31660739) （201610758124）
Key Project of Scientific Research Program of the Higher Education Institution of Xinjiang (XJEDU2016I021) （XJEDU2016I021）
Undergraduate Innovation Project of Xinjiang Agricultural University (201610758124) （201610758124）
