世界中医药2018，Vol.13Issue(2)：252-255,4.DOI:10.3969/j.issn.1673-7202.2018.02.003
羚羊角塞特异性PCR鉴别方法研究
Molecular Authentication of Saigae Tataricae Cornu Tampon by Specific PCR
摘要
Abstract
Objective:To solve the problem of authentication difficulty of saigae tataricae cornu tampon due to loss of traditional i -dentify features such as"Tongtianyan",and to establish an accurate authentication method for saigae tataricae cornu tampon. Methods:Specific PCR primers for S.tatarica were designed based on cytochrome coxidase I(COI)sequences and the annealing temperature of PCR reaction conditions was optimized,which was performed to authenticate saigae tataricae cornu tampon and its 7 adulterants.Results:Only saigae tataricae cornu tampon could obtain a ~300 bp specific band in electrophoresis while the adulter-ants did not when the temperature was 64 ℃and 33 cycle number.Conclusion:Specific PCR could accurately and effectively au-thenticate the biological origin of sample is S.tatarica or not.关键词
特异性PCR/分子鉴定/羚羊角塞/中药鉴定Key words
Specific PCR/Molecular authentication/Saigae tataricae cornu tampon/TCM authentication分类
医药卫生引用本文复制引用
蒋超,金艳,袁媛,黄璐琦,赵玉洋..羚羊角塞特异性PCR鉴别方法研究[J].世界中医药,2018,13(2):252-255,4.基金项目
中医药行业科研专项(201407003)——常用大宗中药材质量现场快速检测技术研究 （201407003）
中央级公益性科研院所基本科研业务费专项资金资助(ZZ10-008) （ZZ10-008）
