世界中医药2018，Vol.13Issue(2)：415-419,5.DOI:10.3969/j.issn.1673-7202.2018.02.042
玉米须总皂苷通过调节炎性反应因子TNF-α和IL-6改善2型糖尿病大鼠脂代谢
Improvement of Lipid Metabolism of Type 2 Diabetes Rats by Zea Mays L.Saponin(ZMLS) Through Regulating Tumor Necrosis Factor-α(TNF-α)and InterLeukin-6(IL-6)
摘要
Abstract
Objective:To study the effects of Zea mays L.saponin(ZMLS)on type 2 diabetes rats induced by high fat diet and Streptozocin(STZ),which provided a fundamental basis for the treatment of the disease.Methods:A total of 60 male SD rats were randomly divided into normal group,type 2 diabetes group(model groups),and ZMLS group,with 20 cases in each group.Rats in ZMLS group were given ZMLS 4 mg/kg/day by gavage treatment,rats in normal group and model group were given physiological sa-line by gavage.After treatment for 4 weeks and 8 weeks,half of the rats were sacrificed,HE staining was used to detect the Liver fat accumulation characteristics,and lipid and antioxidant indexes were detected by the kit.The expression of TNF-αand IL-6 in liver tissue were detected by RT-PCR and Western Blot.Results:HE staining showed that there were obvious fat vacuoles in the model group compared with the normal group,which showed an obvious abnormality of liver fat metabolism in type 2 diabetes rats.After treatment,the fat vacuoles decreased significantly.Compared with the normal group,the blood sugar,TG and MDA in the model group were significantly increased(P<0.05); insulin,HDL-C and SOD were decreased(P<0.05).After 4 weeks of ZMLS treatment,the levels of TG and MDA were significantly decreased(P<0.05)and the levels of insulin,HDL-C and SOD were sig-nificantly increased(P<0.05),which further improved after 8 weeks of treatment(P<0.05);Compared with the normal group by RT-PCR and Western Blot,the expression of TNF-αand IL-6 in the model group was significantly increased(P<0.05)and then decreased after 4 weeks treatment,but the improvement was not obvious.After 8 weeks of treatment,all the indexes significant-ly reduced(P<0.05).Conclusion:ZMLS can significantly regulate blood lipid of rats with type 2 diabetes,which maybe related with its regulating function of the expression of TNF-αand IL-6.关键词
玉米须总皂苷/肿瘤坏死因子-α/白介素-6/2型糖尿病/大鼠Key words
Zea mays L.saponin(ZMLS)/Tumor Necrosis Factor-α/InterLeukin-6/Type 2 Diabetes/Rat分类
医药卫生引用本文复制引用
余渊,程杰..玉米须总皂苷通过调节炎性反应因子TNF-α和IL-6改善2型糖尿病大鼠脂代谢[J].世界中医药,2018,13(2):415-419,5.基金项目
云南省应用基础研究计划项目(2015HS048) （2015HS048）
