世界中医药2018，Vol.13Issue(2)：460-463,4.DOI:10.3969/j.issn.1673-7202.2018.02.053
高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷的含量
Contents of Astragaloside in Huangqi Capsules Measured by HPLC-ELSD
摘要
Abstract
Objective:To evaluate contents of astragaloside in Huangqi Capsules measured by HPLC -ELSD.Methods:The octade-cyl silane bonding silica gel was the filler,with acetonitrile-water(32:68)as mobile phase,and the detector was ELSD with the column pressure of 11.2~12.3 MPa and the flow rate of 1.0 mL/min.Column temperature:indoor temperature.Theoretical plate number was calculated by puerarin peak which should not be less than 4 000; Trailing factor was 1.02.Results:The method of HPLC-ELSD had good accuracy,repeatability,stability and recovery rate.A good linearity relationship of the sample size of asre-agaloside comparison products in the range of 1.0528-10.528 μg(r=0.999 9,n=7).Conclusion:Contents of astragaloside in Huangqi Capsules measured by HPLC-ELSD is feasible.关键词
黄七胶囊/高效液相色谱-蒸发光散射检测器法/黄芪甲苷Key words
Huangqi Capsules/HPLC-ELSD/Astragaloside分类
医药卫生引用本文复制引用
杜鹏,顿宝生,杨新杰,彭修娟..高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷的含量[J].世界中医药,2018,13(2):460-463,4.基金项目
咸阳市2014年重大科技专项(2014k01-21) （2014k01-21）
