山西农业大学学报（自然科学版）2017，Vol.37Issue(12)：866-871,6.
β防御素104a在不同日龄雄性山羊睾丸与附睾中的定位及表达特性
Localization and expression character of Beta-Defensins 104a in different days of male goat testis and epididymis
摘要
Abstract
[Objective]This study was conducted to investigate the protein expression level of gBD104a in testis and epididymis of Jinlan Cashmere Goat at different developmental stages.[Methods]Immunohistochemical staining was used to analyze the location of gBD104a both in testis and epididymis of Cashmere Goat.[Results]Immunohistochemistry result showed that a positive reaction was found in Sertoli cells,spermatogenic cells,spermid and columnar epithelial cells,ciliated cells and luminal fluid of epididymis;The result of immunohistochemistry were analyzed by optical density value showed that the order of the expression level of different parts of epididymis in different development stages was corpus＞caput＞cauda of epididymis,which had a significant difference (P＜0.05).The expression of gBD104a in corpus increased with the days of age,and the expression level of gBD104a in caput reached the peak in 150 days old bucks.The expression of gBD104a cauda had same tendency with that in the testis:the expression level of gBD104a in caput reached the peak in 150 days old bucks.Immunofluorescence result showed that the acrosome of sperm head,neck,and the principal piece of end had positive reaction.[Conclusion]gBD104a differentially expressed in testis and epididymis of goats by the spatio-temporal.specificity.Corpus could be the functional part between gBD104a and spermatogenesis.gBD104a coated in the sperm acrosome site could associate with capacitation or acrosome reaction of sperm,which studies on gBD104a's function still need to be done.关键词
gBD104a/不同发育阶段/免疫组化/山羊Key words
gBD104a/Different developmental stages/Immunohistochemistry/Goat分类
农业科技引用本文复制引用
郭丽娜,张国林,任有蛇,姜玉锁,刘文忠,张春香..β防御素104a在不同日龄雄性山羊睾丸与附睾中的定位及表达特性[J].山西农业大学学报（自然科学版）,2017,37(12):866-871,6.基金项目
山西省科技攻关计划项目(20110311029 （）
20130311025-2) （）
