深圳大学学报（理工版）2018，Vol.35Issue(1)：1-7,7.DOI:10.3724/SP.J.1249.2018.01001
靶向敲除ck2α基因及其功能分析
Target knockout of ck2α gene and its functional analysis
摘要
Abstract
Protein kinase CK2α was knocked out in human colorectal cancer cell line HCT116 by using CRISPR-Cas9 gene editing technique to investigate CK2α's function.PX459-g CK2α vector was successfully transfected into HCT116 cells,and screened gene knockout cell line to further analysis whether it was involved in the proliferation and migration of cancer cells.The results show that HCT116 cell line with ck2α knockout is successfully obtained by CRISPR-Cas9 gene editing technique.ck2α knockout can significantly reduce the cell proliferation rate and migration ability,and accelerate the process of cellular senescence.关键词
基因编辑/CRISPR-Cas9技术/基因敲除/蛋白激酶CK2/细胞增殖/结肠癌/细胞衰老Key words
gene editing/CRISPR-Cas9/gene knockout/protein kinase CK2/cell proliferation/colorectal cancer/cellular senescence分类
医药卫生引用本文复制引用
敖英,张杰,李枫,金美玲..靶向敲除ck2α基因及其功能分析[J].深圳大学学报（理工版）,2018,35(1):1-7,7.基金项目
国家自然科学基金资助项目(81471407) （81471407）
深圳市科技计划项目(JCYJ20140418095735635)National Natural Science Foundation of China(81471407) （JCYJ20140418095735635）
Shenzhen Science and Technology Research Foundation(JCYJ20140418095735635) （JCYJ20140418095735635）
