天然产物研究与开发2018，Vol.30Issue(2)：176-184,231,10.DOI:10.16333/j.1001-6880.2018.2.002
基于UPLC-Q-TOF/MS的归身和归尾补血机制的代谢组学初步研究
Metabolomic Analysis of Enriching Blood Function of Angelica Sinensis Body and Tail Using UPLC-Q-TOF/MS
摘要
Abstract
The aim of the present study was to identify the different endogenous metabolites in hemolytic blood deficiency rats,and subsequently compare the different enriching-blood mechanisms of Angelica sinensis body and Angelica sinensis tail.Angelica sinensis body and Angelica sinensis tail were used to intervene the hemolytic blood deficiency rats induced by N-acetyl phenyl hydrazine (APH).Metabolomic fingerprints of rat serum samples were determined by ultrahigh-pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS).The data sets were then imported and subjected to multivariate statistical analysis and univariate statistical analysis using SIMCA-P14.0.Compared with normal group,the levels of succinate,uracil,orotice acid,cytidine,uridine,cholic acid,pyroglutamate acid,cytosine,deoxyuridine,3-methylglycine,and methyladenosine were significantly (P ＜ 0.05) elevated in model group,while the levels of L-glutamiue,1-tryptophan,isoleucine,PC (16 ∶ 0/16 ∶ 0),and L-prolinein were significantly (P ＜0.05) decreased,indicating the hemolytic blood deficiency model was built successfully.In addition,the abnormal contents of serum metabolites could be turned over after the treatment of Angelica sinensis body and Angelica sinensis tail,and the Angelica sinensis body had better effects.On the basis of these metabolites,it appeared that the pyrimidine,amino acid metabolism,fatty acid metabolism and energy metabolism could be regulated by Angelica sinensis body and Angelica sinensis tail.关键词
归身/归尾/补血机制/代谢组学Key words
Angelica sinensis body/Angelic sinensis tail/enriching blood mechanism/metabolomic分类
医药卫生引用本文复制引用
杨秀娟,邓毅,吴国霞,杨延泽,杨志军,曼琼..基于UPLC-Q-TOF/MS的归身和归尾补血机制的代谢组学初步研究[J].天然产物研究与开发,2018,30(2):176-184,231,10.基金项目
国家自然基金地区科学基金(81360633) （81360633）
甘肃省自然科学基金(1506RJZA044) （1506RJZA044）
甘肃省中管局项目(GZK-2016-25) （GZK-2016-25）
甘肃省教育厅高等学校科研项目(2017A-056) （2017A-056）
甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金(zzy-2016-06) （藏）
