天然产物研究与开发2018，Vol.30Issue(2)：191-198,8.DOI:10.16333/j.1001-6880.2018.2.004
火麻仁植物甾醇含量测定及肠道菌培养对甾醇干预的剂量响应
Determination of Fructus Cannabis Phytosterol and Its Dose-response Relationship to Microflora Intestinal
摘要
Abstract
This paper firstly offered an effective method of determining Fructus Cannabis phytosterol by gas chromatography (GC).The contents of phytosterol of 5 samples were compared.The results showed that the extraction method of sample 2 had high yield,simple and low cost.Through this repeatable method,a more accurate result can be got.Then a study on the dose-response relationship of phytosterol to intestinal microlflora was made.The method of Co-incubation with human intestinal microflora and phytosterol extract was used to detect the dose responses of the growth of phytosterol to intestinal microflora.The results were analyzed by SPSS Statistics 19.0.In vitro experiment of human intestinal microflora:compared with black control group,as the concentration increased from 0.08516 mg/mL to 0.3407 mg/mL,the number of E.coli increased obviously (P ＜ 0.01).Fructus Cannabis extract can promote the proportion of human intestinal microflora positive bacilli in vitro,and can promote the growth of Bifidobacterium.The results showed that phytosterol in Fructus Cannabis can promote the growth of probiotics.关键词
火麻仁甾醇/含量测定/气相色谱法/人源肠道菌群/量效关系Key words
Cannabis semen phytosterol/content determination/gas chromatography/human intestinal flora/dose-response relationship分类
医药卫生引用本文复制引用
李根林,吕宁,吴宿慧,李寒冰,齐月娟..火麻仁植物甾醇含量测定及肠道菌培养对甾醇干预的剂量响应[J].天然产物研究与开发,2018,30(2):191-198,8.基金项目
国家自然基金面上项目(81473435) （81473435）
河南中医学院科技创新人才支持计划(2015XCXRC02) （2015XCXRC02）
