天然产物研究与开发2018，Vol.30Issue(2)：199-206,8.DOI:10.16333/j.1001-6880.2018.2.005
酸化环境对血管平滑肌细胞(VSMC)增殖的影响及姜黄素对此的作用
Effect of Acidification Environment on Proliferation of Vascular Smooth Muscle Cells and the Influence of Curcumin on It
摘要
Abstract
To explore the effect and possible mechanism of curcumin on proliferation of vascular smooth muscle in the acidification environmental caused by ischemic hypoxia.The rats'thoracic aorta VSMC cells were cultured in vitro in pH 6.9,treated with curcumin and PCTX-1 respectively,and the blank control group was set.The expression of ASICS 1a protein and phosphorylation ERK1/2 protein was measured by Western-Blot assay,the effect of each treatment group on the proliferation of VSMC was measured by CCK-8 method.The results showed that the pH 6.9 group can significantly stimulate the expression of ASICS 1a and phosphorylation of ERK1/2 in VSMC and promote the proliferation of VSMC.However,curcumin can significantly inhibit the expression of ASICS 1a and intracellular phosphorylation of ERK1/2 protein in the vascular smooth muscle cells of rats'thoracic aorta,thereby inhibiting the proliferation of VSMC.In the acidification environment caused by ischemia hypoxia,the expression of ASICS 1a protein on rats’ thoracic aortic vascular smooth muscle increased,caused ERK1/2 phosphorylation in cells,managed the proliferation of vascular smooth muscle,while curcumin showed the inhibitory effect.关键词
姜黄素/血管平滑肌细胞/细胞增殖/酸敏感离子通道1a/ERK1/2Key words
curcumin/vascular smoothmuscle cells/cell proliferation/acid-sensing ion channel 1a/extracellular signal-regulated kinase 1/2分类
医药卫生引用本文复制引用
邱韵,李家富,冯健,钟毅..酸化环境对血管平滑肌细胞(VSMC)增殖的影响及姜黄素对此的作用[J].天然产物研究与开发,2018,30(2):199-206,8.基金项目
国家自然科学基金(31300946) （31300946）
泸州市-泸州医学院合作科技项目(2013LZLY-J53) （2013LZLY-J53）
