天然产物研究与开发2018，Vol.30Issue(2)：212-217,6.DOI:10.16333/j.1001-6880.2018.2.007
过墙风总黄酮对四氯化碳致急性肝损伤小鼠的保护作用及机制研究
Protective Effects and Functional Mechanism of Clerodendrum philippinum Schauer var.simplex Mlodenke Total Flavonoids on Acute Liver Injury Induced by Carbon Tetrachloride in Mice
摘要
Abstract
To explore the protective effects and its mechanism of Clerodendrum philippinum Schauer var.simplex Mlodenke total flavonoids (CPTF) on acute liver injury induced by carbon tetrachloride (CCl4) in mice.60 mice were randomly divided into normal group,model group,silymarin group (150 mg/kg),CPTF-high,medium and low dose group (200,100,50 mg/kg).The normal group and model group were given equivoluminal distilled water,and other groups were given the corresponding dose of drugs (25 mL/kg) for 10 days.After 2 h of the last administration,the mice of all groups except the normal group were injected intraperitoneally with 0.1％ CCl4 peanut oil solution (10 mL/kg).16 h later,the blood was taken by removing eye balls,and the liver tissue was collected after killing the mice.The serum contents of alanine aminotransferase (ALT),aspartate aminotransferase (AST),total superoxide dismutase (T-SOD),malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were measured by biochemical method;the levels of tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β) and interleukin-6 (IL-6) in liver tissue were detected by enzymelinked immunosorbent assay (ELISA);janus kinase 2 (JAK2),phosphorylated JAK2 (p-JAK2),signal transducer and activator of transcription 3 (STAT3) and phosphorylated STAT3 (p-STAT3) in liver tissue were detected by Western blot;the histopathological changes of liver were observed by HE staining.The results showed that CPTF could significantly improve the liver tissue lesion,decrease the levels of AST,ALT and MDA in serum (P ＜ 0.05),enhance the activity of T-SOD and GSH-Px (P ＜ 0.05),inhibit the hepatic levels of TNF-α,IL-1β and IL-6 (P ＜ 0.05),and inhibit the expression of p-JAK2 and p-STAT3 (P ＜ 0.05).In summary,CPTF had a protective effect on CCl4-induced acute liver injury in mice,which could reduce the degree of liver injury,and its mechanism may be related to anti-oxidation,inhibition of inflammation and regulation of JAK2/STAT3 signaling pathway.关键词
过墙风/总黄酮/四氯化碳/急性肝损伤/氧化应激/炎症反应/JAK2/STAT3信号通路Key words
Clerodendrum philippinum Schauer var.simplex Mlodenke/total flavonoids/carbon tetrachloride/acute liver injury/oxidative stress/inflammatory response/JAK2/STAT3 signaling pathway分类
生物科学引用本文复制引用
黄思茂,高雅,曹后康,葸博婷,王刚,马冬梅,张可锋..过墙风总黄酮对四氯化碳致急性肝损伤小鼠的保护作用及机制研究[J].天然产物研究与开发,2018,30(2):212-217,6.基金项目
广西八桂学者专项(2017-143) （2017-143）
