天然产物研究与开发2018，Vol.30Issue(2)：225-231,7.DOI:10.16333/j.1001-6880.2018.2.009
闽北林下种植多花黄精的总黄酮含量分析及其生物活性评价
Content Analysis and Bioactivity Evaluation of the Total Flavonoids Extracted from Polygonatum cyrtonema Cultivated under Forest in North Fujian
摘要
Abstract
The suitable conditions for total flavonoids extraction assisted with ultrasonic wave were investigated with the research objects of Polygonatum cyrtonema cultivated under four common types of forest in North Fujian province,the different contents of total flavonoids were ditermined,and their hydroxyl radical scavenging capacities and combination effects with bovine serum albumin were evaluated respectively.The results showed that the suitable conditions for total flavonoids extraction were:ultrasonic power 250 W,ethanol volume fraction 70％,ultrasonic extraction time 20 min,solid-liquid ratio 1∶20 (g∶ mL).Under these optimized conditions,the total flavonoids content of Polygonatum cyrtonema cultivated under moso bamboo forest was the highest which reached (10.30 ±0.05) mg/g;and the total flavonoids content cultivated under natural broad-leaved forest,Cunninghamia lanceolata forest and managed broad-leaved forest was (5.11 ± 0.06),(5.00 ± 0.03) and (5.00 ± 0.05) mg/g respectively.The hydroxyl radical scavenging rate of the total flavonoids reached 89.38％,and owned obvious fluorescence quenching effect by combination with bovine serum albumin,the exponential equation was y =1647 e-0.242x (R =0.9969).关键词
多花黄精/林下种植/总黄酮/羟基自由基清除作用/荧光淬灭能力Key words
Polygonatum cyrtonema/planting under forest/total flavonoids/hydroxyl radical scavenging/fluorescence quenching capacity分类
医药卫生引用本文复制引用
张传海,林志銮,李宝银,华伟平,陈文翰,杨斌..闽北林下种植多花黄精的总黄酮含量分析及其生物活性评价[J].天然产物研究与开发,2018,30(2):225-231,7.基金项目
福建省自然科学基金(2015J01623) （2015J01623）
福建省科技厅高校产学合作重点项目(2016N5011) （2016N5011）
南平市科技计划(N2014Z01) （N2014Z01）
武夷学院引进人才科研启动基金(YJ201401) （YJ201401）
福建省教育厅科技项目(JA15521) （JA15521）
