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增香细菌HD-7的鉴定及香气成分分析

吴丽君 梁开朝 王帆 高锐 王毅 段如敏 刘好宝 张海波 白晓莉

天然产物研究与开发2018,Vol.30Issue(2):246-250,5.
天然产物研究与开发2018,Vol.30Issue(2):246-250,5.DOI:10.16333/j.1001-6880.2018.2.012

增香细菌HD-7的鉴定及香气成分分析

Identification and Key Aroma Compounds Analysis of Strain HD-7

吴丽君 1梁开朝 2王帆 3高锐 1王毅 1段如敏 1刘好宝 2张海波 3白晓莉1

作者信息

  • 1. 云南中烟工业有限责任公司,昆明650202
  • 2. 中国烟草总公司海南省公司海南雪茄研究所,海口571100
  • 3. 中国科学院青岛生物能源与过程研究所,青岛266101
  • 折叠

摘要

Abstract

Aroma-producing microorganisms have considerable promise for producing natural flavors and fragrances in an environment-friendly way.In this study,morphological characterization,physiological and biochemical test,and 16S rD-NA sequence analysis were performed to identify aroma-producing strain HD-7.Additionally,the aroma compositions from aroma-producing strain HD-7 fermentation were analyzed by gas chromatography-mass spectrometry (GC-MS).The result showed that the strain HD-7 was a Bacillus pumilus.A large number of aroma compositions were detected by GC-MS,and the main components included ethyl propionate,acetic acid,n-Propyl acetate,3-hydroxy-2-Butanone with the contents of 25.56%,12.68%,6.80%,4.86%,respectively,among which the ethyl propionate and 3-hydroxy-2-Butanone were widely used as flavors and fragrances.The aroma-producing strain HD-7 is of great application value for production of natural flavors and fragrances.

关键词

产香细菌/短小芽孢杆菌/气相色谱-质谱(GC-MS)联用技术/香气物质/天然香料

Key words

aroma-producing bacteria/Bacillus pumilus/gas chromatography-mass spectrometry/aroma compounds/natural flavors and fragrances

分类

生物科学

引用本文复制引用

吴丽君,梁开朝,王帆,高锐,王毅,段如敏,刘好宝,张海波,白晓莉..增香细菌HD-7的鉴定及香气成分分析[J].天然产物研究与开发,2018,30(2):246-250,5.

基金项目

云南省科技厅项目(2015IA026) (2015IA026)

天然产物研究与开发

OA北大核心CSCDCSTPCD

1001-6880

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