天然产物研究与开发2018，Vol.30Issue(2)：257-260,217,5.DOI:10.16333/j.1001-6880.2018.2.014
超高效液相色谱法同时测定川射干中7个异黄酮成分的含量
Simultaneous Determination of 7 Isoflavone Components in Iris tectorum Using UPLC
摘要
Abstract
To establish a UPLC method for the simultaneous determination of tectoridin,iristectorin A,iristectorin B,iridin,tectorigenin,iristectorigenin A,iristectorigenin B in Iris tectorum Maxim..Waters BEH C18 (2.1 mm × 50 mm,1.7 μm) column was used,with acetonitrile-0.5％ glacial acetic acid as the mobile phases in gradient elution mode.The column temperature was 35 ℃,with flow rate 0.5 mL/min.The UV detection wavelength was set at the maximum absorption wavelength,266 nm for all components.The seven kinds of isoflavone components in I.tectorum were separated well,and the linear relationship was obtained (r ≥0.9990).The average recoveries were 99.68％,100.57 ％,98.43 ％,98.03％,99.14％,97.53％ and 101.86％,with RSDs were 0.84％,1.82％,1.67％,1.54％,0.89％,1.95％ and 1.77％,respectively.The established method was rapid,simple and accurate,it can provide the basis for the quality control of L tectorum.关键词
异黄酮/川射干/质量控制/超高效液相色谱Key words
isoflavone/Iris tectorum Maxim./quality control/UPLC分类
医药卫生引用本文复制引用
陈帅,袁崇均,罗森,王笳..超高效液相色谱法同时测定川射干中7个异黄酮成分的含量[J].天然产物研究与开发,2018,30(2):257-260,217,5.基金项目
四川省应用基础计划(2016JY0009) （2016JY0009）
四川省公益性科研项目(A-2017N-39) （A-2017N-39）
