天然产物研究与开发2018，Vol.30Issue(2)：294-298,245,6.DOI:10.16333/j.1001-6880.2018.2.020
裂果薯皂苷的特征图谱及总皂苷中有效组分的抗肿瘤活性研究
The Characteristic Spectra of Saponins of Schizocapsa Plantaginea Hance and the Antitumor Activity of Effective Components in the Total Saponins
摘要
Abstract
The characteristic spectra of total saponins of Schizocapsa plantaginea Hance (SFSP60％) were established by High Performance Liquid Chromatography with Ultraviolet and Evaporative Light Scattering Detector.Acetonitrile-water mobile phase gradient Elution.The detection wavelength was 203 nm,the column temperature was 30 ℃ and the flow rate was 1 mL/min.Sample volume 10 μL.Reference to the SFSP60％ chromatographic peaks in the ELSD chromatogram.Area normalization method is used to calculate saponin Ⅰ of Schizocapsa plantaginea Hance (SPHS Ⅰ) accounted for 84.28％ (The relative percentage),the saponin Ⅱ of Schizocapsa plantaginea Hance (SPHS Ⅱ) was 3.02％,the compound 1 was 2.21％,the compound 2 was 6.31％,the compound 3 was 2.73％,Compounds 1-3 may be nucleosomal similar saponins.Take the logarithmic growth phase SMMC-7721 cells,cells in the administration group were added to 0,0.25,0.5,2,4 μg/mL SFSP60％,SPHS Ⅰ,SPHS Ⅱ and SPHS Ⅰ + Ⅱ (SPHS Ⅰ and SPHS Ⅱ accounted for 96.54％,3.46％) for 24,48,72 h.MTT assay was used to detect the proliferation inhibition rate of SMMC-7721 cells in order to finally calculate its half inhibitory concentration (IC50).The results showed that the inhibitory effect of the administration group on the proliferation of SMMC-7721 cells was in a time-and dose-dependent manner (all P ＜ 0.05),and the viability size was SFSP60％ ＜ SPHS Ⅰ,SPHS l ＜ SPHS Ⅰ + Ⅱ.It was proved that SPHS Ⅰ and SPHS Ⅱ play the main antitumor effect in total saponins,and the anti-tumor effect is the result of the interaction of each component.Which provided a reference for the study of the viability of effective components in Schizocapsa plantaginea Hance and a new idea for the rational allocation of effective components.关键词
裂果薯/裂果薯总皂苷/裂果薯皂苷特征图谱/裂果薯皂苷Ⅰ/裂果薯皂苷Ⅱ/肿瘤细胞增殖Key words
Schizocapsa plantaginea Hance/total saponins of Schizocapsa plantaginea Hance/characteristics of Saponins of Schizocapsa plantaginea Hance/SPHS Ⅰ/SPHS Ⅱ/tumor cell proliferation分类
化学化工引用本文复制引用
罗舜仁,邱汉琛,陈燕燕,黄修影,廖智红,刘布鸣,梁钢..裂果薯皂苷的特征图谱及总皂苷中有效组分的抗肿瘤活性研究[J].天然产物研究与开发,2018,30(2):294-298,245,6.基金项目
国家自然科学基金(81460620) （81460620）
广西自然科学基金(2014GXNSFDA118025) （2014GXNSFDA118025）
广西中药质量标准研究重点实验室开放课题(201105) （201105）
