天然产物研究与开发2018，Vol.30Issue(2)：299-303,5.DOI:10.16333/j.1001-6880.2018.2.021
荭草花醇提物对H2O2诱导的H9c2细胞氧化损伤的保护作用机制研究
Protective Effect of Flowers of Polygonum orientale Flower Ethanol Extract on H2O2-induced Oxidative Stress Injury in H9c2 Cells
摘要
Abstract
To investigate the protective effect of Polygonum orientale Flower Ethanol Extracton H2O2 induced Oxidative damage in H9c2 Cells.200 μ mol/L H2O2 was used to induce the oxidativestress damage model in H9c2 cells for 0.5 h in vitro.The H9c2 cells were randomly divided into control group,the model group(200 μmol/L H2O2)and the Polygonum orientale Flower ethanol Extract different groups(20、40、80 μg/mL).Cellviability was determined by MTS assay.Thecontentoflactate dehydrogenase (LDH),malondialdehyde (MDA) and the activities of superoxidedimutase (SOD)and catalase (CAT) were detected by biochemical kits.The protein levels of caspase-3,Bax,Bcl-2,p-AKT and AKT were detected by Western blot.Compared with model group,Polygonum orientale Flower Ethanol Extract pre-treatment improved cell viabilityand the activities of SOD and CAT in a dose-dependent manner,and attenuated leakage of LDH and content of MDA.Polygonum orientale Flower Ethanol Extract depressed myocardial apoptosis by down-regulating pro-apoptotic protein cleavedcaspase-3 and Bax,up-regulating apoptosis inhibitory protein Bcl-2 and increasing the protein level of p-AKT.LY294002 could offsets such an effect of Polygonum orientale Flower Ethanol Extract.In summary,Polygonum orientale Flower Ethanol Extract showed a protective effect on H2O2 induced injury in H9c2 cells.This protection may result from inhibiting myocardial oxidative apoptosis,balancing the oxidative stress and maybe related with the PI3K/Akt signaling pathway.关键词
荭草花醇提物/氧化应激损伤/H9c2心肌细胞/PI3K/AktKey words
Polygonum orientale Flower Ethanol Extract/oxidative stress injury/H9c2 cells/PI3 K/Akt分类
医药卫生引用本文复制引用
刘亭,吴琼,刘香香,陆定艳,黄文炫,李月婷,李勇军..荭草花醇提物对H2O2诱导的H9c2细胞氧化损伤的保护作用机制研究[J].天然产物研究与开发,2018,30(2):299-303,5.基金项目
国家自然科学基金(81560630 （）
81760699) （）
贵州省科学技术厅人才团队项目(2016-5613-5677) （2016-5613-5677）
贵州省教育厅项目(2013-04) （2013-04）
