现代妇产科进展2018，Vol.27Issue(2)：90-94,5.DOI:10.13283/j.cnki.xdfckjz.2018.02.002
PRMT5对卵巢癌细胞增殖及侵袭转移能力的影响
Effect of PRMT5 on proliferation,migration and invasion of ovarian cancer cells
摘要
Abstract
Objective:To investigate the effect of protein arginine methyltransferase 5 (PRMT5)-siRNA-mediated gene silencing on proliferation,migration and invasion of ovarian cancer cell line A2780 and SKOV3. Methods:The human PRMT5-siRNA (siP)and controlsiRNA(siC)sequences were chemically synthesized and were transfected into ovarian cancer cell line A2780 and SKOV3 respectively. PRMR5 was down-regulated successfully. BrdU incorporation was used to detect the proliferation activity and transwell assay was employed to test migration and invasion of ovarian cancer cells after siP was transfection. E-cadherin protein was detected by Western blot. Results:PRMT5 protein was down-regulated significantly after siP transfected in ovarian cancer cells. Compared to control sample(siC),cells treated with siP showed significantly decreased BrdU incorporation,in both A2780 and SKOV3 (P<0. 001,respectively).In migration and invasion transwell assays,the number of cells with siP group was lower than that of siC and N groups (P<0. 001,respectively). E-cadherin protein was up-regulated after siP transfection in ovarian cancer cells after 72h. Conclusion:PRMT5-siRNA could significantly inhibit the proliferation,migration and invasion in ovarian cancer cell A2780 and SKOV3,suggest that PRMT5 could play an important role in the tumorigenesis and development of ovarian cancer. Maybe,PRMT5 was involved in migration and invasion of ovarian canser cells via the expression of E-cadherin.关键词
卵巢癌/精氨酸甲基转移酶5/小干扰RNA/生长增殖/迁移/侵袭Key words
Ovarian cancer/Protein arginine methyltransferase 5(PRMT5)/siRNA/Poliferation/Migration/Invasion分类
医药卫生引用本文复制引用
包香香,张瑞,王子超,李红,陈芳..PRMT5对卵巢癌细胞增殖及侵袭转移能力的影响[J].现代妇产科进展,2018,27(2):90-94,5.基金项目
山东省医药卫生科技发展计划项目(No:2016WS0653) （No:2016WS0653）
