现代妇产科进展2018，Vol.27Issue(2)：103-107,5.DOI:10.13283/j.cnki.xdfckjz.2018.02.005
高强度聚焦超声经线粒体途径诱导在体子宫肌瘤细胞发生凋亡的机制研究
Research of the mechanism of high intensity focused ultrasound in inducing apoptosis of invivo uterine myoma cell through mitochondrial pathway
刘珊珊 1白军 1杨斌健 1罗新1
作者信息
- 1. 暨南大学附属第一医院妇产科,广州 510632
- 折叠
摘要
Abstract
Objective:To observe and detect the cell morphology changes,the altera-tions in apoptosis rate of myoma cell and the differences in apoptin expression of invivo uterine myoma treated by High Intensity Focused Ultrasound(HIFU). To explore the effective mecha-nism of HIFU from the angulation of apoptosis. Methods:Three specimens were taken from the ablation group,the surrounding of the ablation group and no treatment group. To observe the cell morphology changes of each group under optical microscopy and electron microscope. Annexin V-FITC / PI double staining flow cytometry was performed to detect. To compare the alterations in apoptosis rate of myoma cell among the ablation group,the surrounding of the ablation group and no treatment group. Western blot was used to detect the differences in protein expressions of Bax,Bcl-2 and Caspase-3 among the ablation group,the surrounding of the ablation group and no treatment group. Results:Coagulative necrosis were detected in ablation group,apoptosis signs were seen in the surrounding of the ablation group,no abnormal changes occurred in no treatment group. Compared with the ablation group and no treatment group,the apoptosis rate of myoma cell in the surrounding of ablation group was increased,the difference between groups was statistically significant (P < 0. 05). Compared with the ablation group and no treatment group,the protein expressions of bax and caspase-3 in the surrounding of ablation group was in-creased,the difference was statistically significant(P<0. 05). The protein expression of Bcl-2 inno treatment group was increased compared to ablation group and the surrounding of ablation group,but the difference between groups was no statistically significant(P>0. 05). Conclusion:After the invivo myoma was ablated by HIFU,coagulative necrosis was caused in the ablation group and apoptosis of myoma cell was induced in the surrounding of ablation group. The possi-ble mechanism may be high expression of Bax and caspase-3 protein was induced in the sur-rounding of ablation group which mediated apoptosis of myoma cell through the mitochondria pathway.关键词
子宫肌瘤/高强度聚焦超声/凋亡/Bcl-2/Bax/线粒体Key words
Uterine myoma/High intensity focused ultrasound/Aapoptosis/Bcl-2/Bax/Mitochondrion分类
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刘珊珊,白军,杨斌健,罗新..高强度聚焦超声经线粒体途径诱导在体子宫肌瘤细胞发生凋亡的机制研究[J].现代妇产科进展,2018,27(2):103-107,5.
