现代妇产科进展2018，Vol.27Issue(2)：112-114,3.DOI:10.13283/j.cnki.xdfckjz.2018.02.007
子宫肌瘤患者MED12高频突变对细胞增殖、侵袭、迁移和凋亡的影响
The effect of high frequency mutation of MED12 on cell proliferation,invasion,migra-
tion and apoptosis in patients with uterine leiomyomas
吴娟 1张子宇 2邹阳 2罗勇 2黄欧平1
作者信息
- 1. 江西省妇幼保健院 妇科,南昌 330006
- 2. 江西省妇幼保健院 中心实验室,南昌 330006
- 折叠
摘要
Abstract
Objective:By using cell proliferation,invasion,migration and apoptosis to study whether the MED12 gene mutation can promote the tumorigenesis of ULs in vitro. Meth-ods:The recombinant plasmid was transfected into 293T cells. Using Western blot to detect MED12 protein expression. CCK-8 method was used to detect cell survival rate. Transwell was used to detect cell migration and invasion. Flow cytometry was used to detect cell apoptosis. Re-sults:Using CCK-8 assay to test cell proliferation,MED12 mutations G44D promoted survival of 293T cells compared with wide-type plasmid and had significant statistical difference (P <0. 01). However,migration,invasion ability,cell cycle and apoptosis had no statistical differ-ence. Conclusion:MED12 may promote the tumorigenesis of ULs through influencing cell pro-liferation.关键词
子宫肌瘤/中介体复合物亚基12/基因突变/细胞增殖Key words
Uterine leiomyoma/MED12/Gene mutation/Cell proliferatio分类
医药卫生引用本文复制引用
吴娟,张子宇,邹阳,罗勇,黄欧平..子宫肌瘤患者MED12高频突变对细胞增殖、侵袭、迁移和凋亡的影响[J].现代妇产科进展,2018,27(2):112-114,3.
