新疆农业科学2017，Vol.54Issue(11)：1999-2005,7.DOI:10.6048/j.issn.1001-4330.2017.11.005
干旱胁迫棉花转录组DNA损伤修复相关基因的分析
Analysis of DNA Damage Repair Related Genes in Drought Stress Cotton Transcriptome
摘要
Abstract
[Objective] The study aims to investigate the expression of DNA damage repair related genes in cotton under drought stress,and to explore the correlation between the expression of DNA damage repair related genes and drought resistance at the whole level.[Method] 2.5％ PEG 6000 was used to treat cotton,and transcriptome was sequenced using RNA-Seq technology of cotton seedlings under drought stress.[Result]A total of 51 genes with differentially expressed DNA damage were filtered from the transcripts of cotton drought stress response,among which there were 23 differentially up-regulated expressed genes and 28 differentially down-regulated expressed genes,which showed that drought stress could affect the expression of DNA repair related genes in cotton.4 genes were selected by bioinformatics analysis and verification results showed that the expression of qRT-PCR,HMGB1,recA1,UDGs amplitude and GMP synthase gene displayed some differences,but the gene expression trends were consistent,showing that cotton transcriptome sequencing results verified by qRT-PCR were reliable.[Conclusion] The DNA damage repair related genes may be related to drought stress,so drought stress could affect the expression of DNA damage related genes.关键词
棉花/干旱胁迫/转录组测序/DNA损伤修复相关基因/差异表达Key words
cotton/drought stress/transcriptome sequencing/DNA damage repair related gene/differential expression分类
农业科技引用本文复制引用
包秋娟,张丽丽,海那尔·乌拉孜巴依,张富春..干旱胁迫棉花转录组DNA损伤修复相关基因的分析[J].新疆农业科学,2017,54(11):1999-2005,7.基金项目
Joint Key Fund of National Natural Science Foundation of China and Xinjiang "Exploration of key genes related to high-efficiency water utilization in cotton and creation of drought resistant germplasm" (U1303282) （U1303282）
国家自然科学基金-新疆联合基金重点项目“棉花水分高效利用的关键基因的挖掘及抗旱种质材料的创制”(U1303282) （U1303282）
