新疆农业科学2017，Vol.54Issue(11)：2046-2053,8.DOI:10.6048/j.issn.1001-4330.2017.11.011
基于分子检测新疆东部棉花根茎部病害种类及其分布
Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection
摘要
Abstract
[Objective] Clarifying species and distribution of cotton root and stem diseases in eastern Xinjiang can provide a basis for effective prevention and control of such diseases in the local area.[Method] The reported specific primers of the 4 species pathogens (Fusarium oxysporum,Rhizoctonia solani,verticillium dahliae and Fusarium verticilliodes) which cause the root and stem disease of cotton are used for the specific verification,and carrying out PCR detection for the mixed disease samples of the collected according to different villages (townships) from the cotton areas in Eastern Xinjiang.[Result] In the tested system with sensitivity of 0.5 ng/μL,3 species of 4 pathogenic bacteria were detected from the 22 mixed samples of cotton infected plants from the Eastern Xinjiang.They were respectively Fusarium oxysporum,Rhizoctonia solani and verticillium dahliae;Among them,Fusarium oxysporum had the highest detection rate,Rhizoctonia solani was the second,V.dahliae dad the lower detection rate.But,the detection rate ofFusarium verticilliodes was 0％ in the 22 mixed samples.[Conclusion] The detection results indicated that the Fusarium wilt,Rhizoctonia damping-off and Verticillium yellow wilt were the main root and stem diseases of cotton in Eastern Xinjiang;Among which,Fusarium wilt is the most common one,followed by Rhizoctonia damping-off,and the incidence of Verticillium wilt is relatively low.In Eastern Xinjiang,red rot has not occurred or distribute very little.关键词
棉花/根茎部病害/PCR检测/病害分布Key words
cotton/root and stem disease/PCR detection/distribution of disease分类
农业科技引用本文复制引用
马依努尔·米吉提,族米娜,萨吉达·艾孜则,郭庆元..基于分子检测新疆东部棉花根茎部病害种类及其分布[J].新疆农业科学,2017,54(11):2046-2053,8.基金项目
Special projects of Ministry of Agriculture "Research and integration of crop root rot disease control technology" (201503112) （201503112）
Key research and development plans of the xinjiang uygur autonomous region "Development of new technology and product for control Main diseases and pests and reduce Pesticide and increase effect in cotton field" (2016B01001) （2016B01001）
农业部专项“作物根腐类病害防控技术研究与集成”(201503112) （201503112）
新疆维吾尔自治区重点研发计划“棉田主要病虫防治及农药减施增效新技术与产品研发”(2016B01001) （2016B01001）
