新疆农业科学2017，Vol.54Issue(11)：2060-2066,7.DOI:10.6048/j.issn.1001-4330.2017.11.013
石河子地区棉花苗期叶斑病病原菌种类鉴定
Identification of Pathogens Causing Leaf Spot Disease at Cotton Seedling Stage in Shihezi Area
摘要
Abstract
[Objective] The aim of the study is to verify the pathogens that cause cotton seedling leaf spot in Shihezi area.[Method] 120 samples of cotton leaf spot were collected from Shihezi area,and 105 strains of Ahernaria spp were isolated and purified by conventional method,and 15 representative strains were identified through survey of morphological characteristics,test of pathogenicity and sequence analysis of histone 3 gene.[Result]The selected 15 representative strains were pathogenic.The pathogens were preliminarily identified as A.alternata and A.tenuissima according to morphological characteristics,respectively.Based on histone 3 gene sequence analysis,the results showed that the similarity of nucleotide homogeneity of A.alternata (AF404620,KF997067 and KR866858) was 99％ and A.tenuissima (KT384348) was more than 99％,respectively.[Conclusion] The pathogens causing cotton seedling leaf spot are A.alternata and A.tenuissima in Shihezi in 2016.关键词
棉苗叶斑病/病原菌/鉴定Key words
cotton seedling leaf spot/pathogen/identification分类
农业科技引用本文复制引用
李映程,张国丽,杨超,任毓忠,李卓,张莉,李国英..石河子地区棉花苗期叶斑病病原菌种类鉴定[J].新疆农业科学,2017,54(11):2060-2066,7.基金项目
Selection of High-yield and Fine-quality Peanuts Varieties and Study on the Cultivation (2016B01008-2) （2016B01008-2）
科技部中小企业创新项目(14C26216513812) （14C26216513812）
