新疆农业科学2017，Vol.54Issue(11)：2067-2074,8.DOI:10.6048/j.issn.1001-4330.2017.11.014
不同产地及品种大蒜内生菌群落多样性分析及拮抗作用测定
Diversity of the endophytes and screening of antagonistic microbes in Garlic varieties from different producing areas
摘要
Abstract
[Objective] In order to investigate diversity of the endophytes in Garlic varieties from different producing area,and obtain antagonistic microbes.[Method] Garlic varieties,white garlic and red garlic from different producing areas were sampled.And then,Biolog Eco method was employed to investigate metabolic characteristics of endophytes.Meanwhile,Endophytes were isolated and antagonistic bacteria to Rhizoctonia soleni,Fusarium oxysporumf,Penicillium expansum,Escherichia coli and Staphyloccocus aureus were screened.[Result] Low metabolic activity of endophytes in Garlic were determined,and some differences in the utilization and diversity in Garlic varieties from different producing areas were analyzed.45 endophytes were isolated which were involved in 15 species belonging to genus Bacillus,genus Paenibacillus,genus Streptomyces,genus Nocardiopsis,and genus Dietz.Isolates in Genus Bacillus were dominant in Garlic endophytes,accounted for more than 85％,but there were differences in the distribution of species.Additionally,lots of antagonistic bacteria were obtained.[Conclusion] Diversity of the endophytes in Garlic varieties from different producing area were all poor,but effective resource of antagonistic microbes needs further explore.关键词
大蒜品种/不同产地/内生菌/拮抗菌Key words
Garlic varieties/different producing areas/endophytes/antagonistic microbes分类
农业科技引用本文复制引用
楚敏,张丽娟,刘晓静,朱静,王玮,顾美英,谢玉清,张志东..不同产地及品种大蒜内生菌群落多样性分析及拮抗作用测定[J].新疆农业科学,2017,54(11):2067-2074,8.基金项目
Natural Science Funds of Xinjiang Uygur Autonomous Region (No.2014211B033) （No.2014211B033）
新疆维吾尔自治区自然基金项目“新疆大蒜根腐病病原菌分离鉴定及发病规律动态分析”(2014211B033) （2014211B033）
