烟草科技2018，Vol.51Issue(1)：1-7,7.DOI:10.16135/j.issn1002-0861.2017.0138
烟草Nt14-3-3基因的克隆及生物信息学和表达模式分析
Cloning,bioinformatics and expression pattern analysis of Nt14-3-3
摘要
Abstract
To study the function of Nt14-3-3 gene family in tobacco (Nicotiana tabacum), Nt14-3-3 was cloned from cv. K326 by homologous cloning. In addition, bioinformatics analysis of Nt14-3-3 was carried out. The transcriptional expressions of Nt14-3-3 were analyzed by qRT-PCR in different tissues of tobacco seedlings under abiotic stresses. The results showed that the gene sequence contained 783 bp in length, and encoded 260 amino acid residues. Nt14-3-3 was a globular protein contained nine highly conserved α helixes and 32 phosphorylation sites. Tissue expression analysis showed that Nt14-3-3 was expressed in tobacco roots, stems, leaves and flowers, especially in roots. The expression levels of Nt14-3-3 were induced under abiotic stress treatments (low potassium, high salinity, drought, ABA and H2O2), which indicated that Nt14-3-3 was involved in tobacco abiotic stress response.关键词
烟草/Nt14-33蛋白/Nt14-3-3基因/基因表达/非生物胁迫Key words
Tobacco/Nt14-3-3 protein/Nt14-3-3/Gene expression/Abiotic stress分类
轻工纺织引用本文复制引用
陈倩,卓维,李佳皓,鲁黎明,李立芹..烟草Nt14-3-3基因的克隆及生物信息学和表达模式分析[J].烟草科技,2018,51(1):1-7,7.基金项目
国家自然科学基金项目"E3(泛素连接酶)参与植物响应低磷胁迫的分子机制"(31070244). （泛素连接酶）
