烟草科技2018，Vol.51Issue(1)：21-27,7.DOI:10.16135/j.issn1002-0861.2017.0356
快中子辐射诱变K326突变体库的创建及初步分析
Construction and analysis of K326 mutant library by fast neutron irradiation mutagenesis
摘要
Abstract
To study the mutagenic effects of fast neutron irradiation(FNI)on tobacco traits and to investigate the feasibility of constructing a flue-cured tobacco mutant library with mutagenesis method, the effects of FNI at different doses(within the range of 5-20 GY)on the germination rate, seedling growth and seedling rate of K326 were studied. The results showed that: 1)FNI was highly efficient in causing mutation in tobacco, and various mutants were obtained. It was a feasible method to construct a mutant library. 2)The appropriate dose of FNI for K326 seeds ranged from 10 to 15 GY. 3)The agronomic traits of fast neutron-mutagenized populations were various, and the populations could be used to study functional genomics or tobacco breeding as germplasm resources. Thus, FNI is an effective, reliable and rapid method for constructing a tobacco mutant library.关键词
快中子/诱变/突变体库/烟草/K326/性状/变异Key words
Fast neutron/Mutagenesis/Mutant library/Tobacco/K326/Trait/Variation分类
农业科技引用本文复制引用
平文丽,俎焕新,李旭辉,侯咏,王燃,金立锋,张小全,李正风,李雪君,张林,孙计平,孙焕,丁燕芳..快中子辐射诱变K326突变体库的创建及初步分析[J].烟草科技,2018,51(1):21-27,7.基金项目
河南省科技攻关项目"基于原生质体再生技术的CRISPR/Cas9技术体系构建"(172102110153) （172102110153）
中国烟草总公司烟草基因组计划重大专项项目"利用镉转运基因创制云烟87低镉品系"[110201601031(JY-05)] （JY-05）
河南省科技攻关项目"利用花药培养创制烤烟抗黑胫病新种质的研究"(142102110164). （142102110164）
