烟草科技2018，Vol.51Issue(1)：43-49,7.DOI:10.16135/j.issn1002-0861.2017.0255
UPLC-HRMS法测定无烟气烟草制品中的小分子醛
Analysis of small-molecule aldehydes in smokeless tobacco products by UPLC-HRMS
摘要
Abstract
To investigate the contents of small-molecule aldehydes in smokeless tobacco products, such as formaldehyde, acetaldehyde, propionaldehyde, acrolein, butyraldehyde and crotonaldehyde, these targets and their internal standards were extracted under ultrasound and derived with 2,4-dinitrophenylhydrazine(DNPH) simultaneously by a water-isohexane biphase system. The derivatives were then analyzed by Q-Exactive mass spectrometry with ultra-performance liquid chromatography-high resolution mass spectrometry(UPLC-HRMS) method. The result showed that: 1)Preparation time was less than 30 min after optimization, and analysis time was reduced to 6 min by adopting C18UPLC column. 2)The interferences of acetone and butanone to propionaldehyde and butyraldehyde were excluded by using characteristic secondary ion for quantitative analysis. 3)The linear ranges of analytes were over two orders of magnitude with good correlation coefficients (R2>0.99), the limits of quantification ranged from 0.07 to 0.90 ng/mL, and the recoveries were in the range of 81.1%-107.6% with relative standard deviations no more than 12.7%. 4)Formaldehyde and acetaldehyde were major small-molecule aldehydes in smokeless tobacco products. The established method is simple, rapid and reliable, it is suitable for the analysis of small-molecule aldehydes in smokeless tobacco products.关键词
无烟气烟草制品/小分子醛/2,4-二硝基苯肼/超声提取/超高效液相色谱-高分辨质谱联用(UPLC-HRMS)Key words
Smokeless tobacco product/Small-molecule aldehyde/DNPH/Ultrasound extraction/Ultra-performance liquid chromatography-high resolution mass spectrometry(UPLC-HRMS)分类
轻工纺织引用本文复制引用
王丁众,孙世豪,宗永立,张建勋,李鹏,张启东,徐秀娟,霍现宽,范武,姬凌波,刘俊辉,毛健..UPLC-HRMS法测定无烟气烟草制品中的小分子醛[J].烟草科技,2018,51(1):43-49,7.基金项目
中国烟草总公司郑州烟草研究院院长科技发展基金项目"无烟气烟草制品中小分子醛酮化合物的检测"(412014CA0370). （412014CA0370）
