烟草科技2018，Vol.51Issue(2)：1-8,8.
利用CRISPR/Cas9技术的烟草NtLS基因敲除分析
Targeted mutagenesis of NtLS gene using CRISPR/Cas9 system
摘要
Abstract
To breed new tobacco varieties with no or less axillary buds, based on CRISPR/Cas9 system, the DNA sequence of NtLS was chosen as the target site for designing two CRISPR/Cas9 vectors (cLS1 and cLS2). Agrobacterium tumefaciens-mediated transformation of cv. K326 was used and twenty kanamycin-resistance tobacco plants (T0generation) were obtained. Ten positively transformed tobacco plants were analyzed by PCR amplification, sequencing and alignment, and some mutations of NtLS protein were detected in two plants. These research results provide useful mutants to study the molecular mechanism of axillary bud formation and create genetic materials for new flue-cured tobacco varieties with no or less axillary buds.关键词
NtLS基因/CRISPR/Cas9/基因组编辑/腋芽Key words
NtLS gene/CRISPR/Cas9/Genome editing/Axillary bud分类
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王姗姗,杨军,王中,林福呈,潘婷,武明珠,张剑锋,曹培健,谢小东..利用CRISPR/Cas9技术的烟草NtLS基因敲除分析[J].烟草科技,2018,51(2):1-8,8.基金项目
中国烟草总公司郑州烟草研究院院长科技发展基金项目"烟草多基因CRISPR/Cas9编辑体系构建及应用研究"(902017CA0210) （902017CA0210）
中国烟草总公司烟草基因组计划重大专项项目"烟草基因功能元件全景图构建及关键基因挖掘与调控节点研究"(902016AA0390). （902016AA0390）
