烟草科技2018，Vol.51Issue(2)：9-15,7.
烟草黑胫病菌对氟吗啉的敏感性检测及复配药剂的效果评价
Study on sensitivity of Phytophthora nicotianae to flumorph and combinatory fungicide
摘要
Abstract
To investigate the effects of Phytophthora nicotianae to flumorph and delay the development of its flumorph resistance, the sensitivity of P. nicotianae to flumorph was determined by a colony growth rate method. In addition, the effects of Phytophthora nicotianae on a combinatory fungicide (flumorph+metalaxyl) were evaluated by the Wadley method. The combination ratio of the fungicide with a high antibacterial activity was selected and the synergistic effects of the flumorph and metalaxyl combination and Bacillus amyloliquefaciens ZY-9-13 were evaluated by the Abbott model. The results showed that the EC50values of 71 strains of P. nicotianae to flumorph ranged from 0.428 5 to 0.760 8 μg/mL with a mean of 0.588 4 μg/mL. The sensitivity distribution was unimodal, and there was no resistant isolate in the 71 strains. The additive effect of the combinatory fungicide at a volume ratio of 1:10 was the most effective (EC50=0.398 7 μg/mL). The growth of B. amyloliquefaciens was not affected by the fungicide. The combined application of the fungicide at different concentrations and ZY-9-13 (1010cfu/mL) had better inhibitory effects comparing with a single application of each fungicide, and there existed a synergistic effect between them.关键词
烟草黑胫病菌/氟吗啉/甲霜灵/解淀粉芽孢杆菌/敏感性/复配药剂Key words
Phytophthora nicotianae/Flumorph/Metalaxyl/Bacillus amyloliquefaciens/Sensitivity/Combinatory fungicide分类
农业科技引用本文复制引用
牟文君,宋纪真,胡利伟,刘芳,薛超群,戴华鑫,陈钊,黄中乔,张志高,奚家勤..烟草黑胫病菌对氟吗啉的敏感性检测及复配药剂的效果评价[J].烟草科技,2018,51(2):9-15,7.基金项目
中国烟草总公司郑州烟草研究院院长科技发展基金项目"烟草黑胫病菌对烯酰吗啉的抗性机制研究"(122015CA0190) （122015CA0190）
中国烟草总公司河南省公司科技项目"烟草黑胫病防控关键技术研发与应用"(122013DS0320) （122013DS0320）
江西省烟草公司抚州市公司项目"烟草青枯病和黑胫病检测方法建立与试剂盒研制"(122015DS0180). （122015DS0180）
