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基于第三代测序技术的基因组组装方法及其在烟草中的应用

卢鹏 金静静 李泽锋 曹培健 范楷 许亚龙

烟草科技2018,Vol.51Issue(2):87-94,8.
烟草科技2018,Vol.51Issue(2):87-94,8.

基于第三代测序技术的基因组组装方法及其在烟草中的应用

Genome assembly based on the third-generation sequencing technology and its application in tobacco

卢鹏 1金静静 1李泽锋 1曹培健 1范楷 1许亚龙1

作者信息

  • 1. 中国烟草总公司郑州烟草研究院,郑州高新技术产业开发区枫杨街2号 450001
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摘要

Abstract

The third-generation sequencing technology has been widely used in genome research due to its advantage of long-fragment reads. The development of the sequencing technology was reviewed and the advantages and disadvantages of three different generations of sequencing technologies were summarized. Based on the third-generation sequencing, the methods of genome assembly including data pre-processing, sequence assembly, sequence repairing after assembly and chromosome localization methods were tracked and statistically analyzed. Application of sequencing technology and genome assembly in tobacco genome research was introduced.

关键词

第三代测序/基因组/组装/烟草

Key words

Third-generation sequencing/Genome/Assembly/Tobacco

分类

生物科学

引用本文复制引用

卢鹏,金静静,李泽锋,曹培健,范楷,许亚龙..基于第三代测序技术的基因组组装方法及其在烟草中的应用[J].烟草科技,2018,51(2):87-94,8.

基金项目

中国烟草总公司郑州烟草研究院院长科技发展基金项目"利用三代测序数据填补基因组缺失序列的方法研究"(902016CA0170). (902016CA0170)

烟草科技

OA北大核心CSCDCSTPCD

1002-0861

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