疑难病杂志2018，Vol.17Issue(3)：277-281,5.DOI:10.3969/j.issn.1671-6450.2018.03.015
百里醌联合5-氟尿嘧啶对胃癌HGC-27细胞增殖和凋亡的影响
Proliferation and apoptosis induced by thymoquinone and 5 fluorouracil on gastric cancer HGC-27 cells
摘要
Abstract
Objective To investigate the proliferation and apoptosis induced by thymoquinone(TQ) combine with 5 fluorouracil(5-Fu) on gastric cancer HGC-27 cells. Methods We conducted the experiment in central laboratory of Renmin Hospital of Wuhan University since September 2016. HGC-27 cells were treated with 50 μmol/L TQ,75 μg/ml 5 Fu or 50 μmol/L TQ + 75 μg/ml 5-Fu,and then the proliferation were detected by CCK-8. Apoptosis was detected by Hoechst stai-ning and Flow cytometry. The protein expressions of Bax and Bcl-2 were detected by Western blot. Results CCK-8 results showed that inhibited exposure to thymoquinone for 24 h clearly inhibited the viability of cells. The inhibition rate of the com-bined group was higher than 5-Fu group and TQ group(F=767.05,P<0.01). Hoechst result showed that the apoptosis rate in the combined group was significantly higher than the 5-Fu. Flow cytometry showed the apoptosis rate of the control group was (2.26 ± 0.17) % and the apoptosis rate of 50 μmol/L TQ,75 μg/ml 5-Fu and 50 μmol/L TQ + 75 ug/ml 5 Fu was (3.76 ± 0.44) %,(6.24 ± 0.19) % and(9.76 ± 0.25) % respectively (F=449.58,P<0.01). The protein expression of Bax was elevated (F= 58.803,P<0.01) and Bcl-2 was decreased (F=67.203, P<0.01) in combined group com-pared with single group. Conclusion Thymoquinone combined with 5 fluorouracil inhibits the proliferation and apoptosis in HGC-27 cell line,and thymoquinone can sensitize 5-Fu mediated growth inhibition.关键词
百里醌/胃癌/HGC-27/增殖/凋亡Key words
Thymoquinone/Gastric cancer/HGC-27 cells/Proliferation/Apoptosis引用本文复制引用
胡雪,马静静,李娇,田山,董卫国..百里醌联合5-氟尿嘧啶对胃癌HGC-27细胞增殖和凋亡的影响[J].疑难病杂志,2018,17(3):277-281,5.基金项目
国家自然科学基金资助项目(81372551) （81372551）
