针刺研究2018，Vol.43Issue(3)：140-145,6.DOI:10.13702/j.1000-0607.170635
电针联合天麻素对阿尔茨海默病大鼠海马CA 1区沉默信息调节因子2同源蛋白1和过氧化物酶体增殖物激活受体 γ 辅激活子1α表达的影响
Electroacupuncture plus Gastrodin Improves Learning-memory Ability Possibly by Up-regulating Expression of SIRT 1 and PGC-1 ɑin Hippocampal CA 1 Region of Alzheimer's Disease Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) combined with Gastrodin on learning-memory ability and expression of silent information regulator 2 homologous protein 1(SIRT 1) and peroxisome proliferator activated recep-tor γ coactivator (PGC-1ɑ) of hippocampal CA 1 region in Alzheimer's disease(AD) rats ,so as to explore its mechanism under-lying improvement of AD .Methods Sixty male SD rats were randomly divided into normal control (normal ) ,sham operation (sham) ,model ,EA ,Gastrodin and EA+ Gastrodin groups (n=10 in each) .TheAD model was established by intraperitoneal in-jection of D-Galactose (120 mg · kg-1 · d-1 ) combined with bilateral hippocampal injection of βamyloid 1-40(Aβ1-40) .EA was applied at "Baihui"(GV 20) ,"Dazhui"(GV 14) and"Zusanli"(ST 36) for 30 min ,once daily for 4 weeks .For rats of the Gastro-din group and EA+ Gastrodin group ,intraperitoneal injection of gastrodin(10 mg/kg) was conducted once daily for 4 weeks .Mor-ris water maze tests were used to assess the rat 's learning-memory ability .Nissl staining was used to assess the morphological changes of neurons in the hippocampal CA 1 area .The expression of SIRT 1 and PGC-1ɑof hippocampal CA 1 region was mea-sured by immunohistochemical staining .Results 1) Morris water maze tests showed that ,compared with the normal and sham group ,the escape latency was significantly prolonged ( P<0 .05) ,and the percentage of platform quadrant residence duration and the platform crossing times were considerably decreased in the model group ( P<0 .05) .After the intervention ,the escape laten-cy was obviously shortened ( P<0 .05) ,and the percentage of platform quadrant residence duration and the platform crossing times were markedly increased in the EA ,Gastrodin and EA+Gastrodin groups relevant to the model group ( P<0 .05) .2) Nissl staining showed that ,in comparison with the normal group or sham group ,the number of cells in the hippocampal CA 1 area was decreased and the arrangement was disorganized in the model group .The number of cells in CA 1 area was relatively higher in the 3 treatment groups than in the model group .3) The expression levels of SIRT 1 and PGC-1ɑproteins in the hippocampal CA 1 area were significantly down-regulated in the model group than in the normal and sham groups ( P<0 .05) .After the intervention ,the expression levels of SIRT 1 and PGC-1ɑin the EA ,Gastrodin and EA+Gastrodin groups were significantly up-regulated compared with the model group (P< 0 .05) .The effects of EA+ Gastrodin were significantly superior to those of simple EA and simple Gastrodin in shortening the escape latency ,up-regulating the expression levels of SIRT 1 and PGC-1ɑas well as in increasing the percentage of platform quadrant residence time and platform crossing times ( P<0 .05) .Conclusion Both EA and Gastrodin can improve the learning-memory ability of AD rats ,which may be related to their effects in up-regulating the expression of SIRT 1 and PGC-1ɑ and reducing neuronal injury in the CA 1 region of hippocampus ,suggesting a protective role of EA on hippocampal neurons .The effect of EA combined with Gastrodin is markedly better than that of EA and Gastrodin alone .关键词
电针/天麻素/阿尔茨海默病/沉默信息调节因子2同源蛋白1/过氧化物酶体增殖物激活受体γ辅激活子/海马CA1区/学习记忆能力Key words
Electroacupuncture/Gastrodin/Alzheimer's disease/Silent information regulator 2 homologous protein 1/Peroxisome proliferator activated receptor γ coactivator/Hippocampal CA 1/Learning-memory ability分类
医药卫生引用本文复制引用
黄锐,吴锋,赵健,李怀斌,丁见,熊克仁..电针联合天麻素对阿尔茨海默病大鼠海马CA 1区沉默信息调节因子2同源蛋白1和过氧化物酶体增殖物激活受体 γ 辅激活子1α表达的影响[J].针刺研究,2018,43(3):140-145,6.基金项目
安徽省自然科学基金项目(No.1608085QH223) （No.1608085QH223）
安徽省熊克仁名师工作室(No.2014msgzs152) （No.2014msgzs152）
安徽省高等学校自然科学研究重点项目(No.KJ2015A227) （No.KJ2015A227）
