针刺研究2018，Vol.43Issue(3)：146-151,6.DOI:10.13702/j.1000-0607.170372
针刺对急性脑缺血大鼠沉默信息调节因子2相关酶1和核转录因子-κB蛋白的影响
Acupuncture Reduces Acute Cerebral Ischemia by Suppressing Local and Peripheral Inflamma-tion and Regulating Expression of Cerebral Protein Silent Information Regulator Factor 2-related Enzyme 1 and Nuclear Factor-κB in Rats
摘要
Abstract
Objective To observe the effect of acupuncture on the expression of silent information regulator factor 2-re-lated enzyme 1 (SIRT 1) and nuclear factor-κB (NF-κB) in rats with acute cerebral ischemia (ACI) ,so as to explore its mecha-nisms underlying improvement of ACI .Methods One hundred female SD rats were randomly divided into 5 groups:normal con-trol (normal ) ,sham-operation (sham) ,model ,non-acupoint and acupoint ,with 20 rats in each .The ACI model was established by occlusion (electric coagulation) of the middle cerebral artery after craniotomy ."Baihui" (GV 20) and"Shuigou" (GV 26) or non-acupoints were punctured with filiform needles which were retained for 30 min after rotating for 1 min .The treatment was con-ducted once after modeling and 24 h thereafter .The cerebral infarct volume was measured after 2 ,3 ,5-triphenyltetrazolium chlo-ride (TTC) staining .The contents of interleukin-1β (IL-1β) ,IL-6 and IL-8 in the serum and ischemic brain tissue were detected by radioimmunoassay ,and the expression of protein SIRT 1 and NF-κB p 65 in the ischemic brain tissue was detected by immunob-lotting .Results The brain infarction volume was obvious in the model group in comparison with the normal group ( P<0 .01) , but was markedly reduced in the acupoint group relevant to the model group ( P<0 .05) .The contents of IL-1β,IL-6 and IL-8 in the serum and ischemic brain tissues , and the expression level of cerebral NF-κB p 65 protein were significantly increased ( P<0 .01) ,and the expression of cerebral SIRT 1 was obviously decreased in the model group compared with the normal group( P<0 .01) .Following acupuncture intervention ,the increased IL-1 β,IL-6 and IL-8 contents and NF-κB p 65 expression were considerably down-regulated ,and the decreased SIRT 1 expression was markedly up-regulated in the acupuncture acupoint group ( P<0 .05) .No significant changes were found in the levels of all the indexes mentioned above between the normal and sham groups ( P>0 .05) .Conclusion Acupuncture intervention at acupoints can reduce the ischemic infarction volume in ACI rats , which may be associated with its effects in down-regulating the levels of IL-1β,IL-6 and IL-8 in the serum and ischemic brain tis-sue ,and in regulating cerebral SIRT 1/NF-κB signaling .关键词
针刺/脑缺血/沉默信息调节因子2相关酶1/核转录因子-κB/炎性细胞因子/脑梗死体积Key words
Acupuncture/Cerebral ischemia/Silent information regulator factor 2-related enzyme 1 (SIRT 1)/Nuclear factor-κB (NF-κB)/Inflammatory cytokines/Cerebral infarct volume分类
医药卫生引用本文复制引用
王琼,黄伟,吴洪阳..针刺对急性脑缺血大鼠沉默信息调节因子2相关酶1和核转录因子-κB蛋白的影响[J].针刺研究,2018,43(3):146-151,6.基金项目
武汉市卫生和计划生育委员会科研项目(No.Z16A) （No.Z16A）
