针刺研究2018，Vol.43Issue(3)：152-162,11.DOI:10.13702/j.1000-0607.170436
基于核磁共振氢谱技术研究电针"内关"穴对心肌缺血再灌注损伤大鼠血清及心肌组织代谢物的影响
Effect of Electroacupuncture at "Neiguan"(PC 6 ) on Serum and Myocardial Metabolites in Rats with Myocardial Ischemia Reperfusion Injury Based on Nuclear Magnetic Resonance Spectroscopy
摘要
Abstract
Objective We have repeatedly demonstrated that electroacupuncture (EA) of "Neiguan"(PC 6) can im-prove myocardial ischemia in rats .The present study was designed to investigate the metabolomic profile of peripheral blood se-rum and myocardium involving EA-induced improvement of myocardial ischemia-reperfusion injury (MIRI) in rats by using nuclear magnetic resonance spectroscopy .Methods Thirty male SD rats were equally randomized into blank control ,model and EA groups .Rats of the control group were only banded for 20 min ,once a day for 7 days .The MIRI model was established by occlu-sion of the anterior descending branch of the left coronary artery for 40 min ,followed by reperfusion for 60 min ,and rats of the model group were banded as those in the control group .EA (10 Hz/50 Hz ,1 mA) was applied to bilateral PC 6 for 20 min ,once daily for 7 days .The blood samples and left ventricular myocardial tissues were collected for assaying the profiles of differential metabolites using 1H nuclear magnetic resonance (1H NMR) spectroscopy and multivariate statistical analysis such as the principal components analysis (PCA) ,partial least squares-discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) with SIM-CA-P software 12 .0 .Results A total of 19 differential metabolites (17 down-regulated ,2 up-regulated) in the serum and 14 dif-ferential metabolites (13 down-regulated and 1 up-regulated) in the ischemic left myocardium were identified after MIRI .Of the 19 serum differential metabolites ,amino acids (leucine ,isoleucine ,valine ,alanine ,lysine ,glycine ,glutamine) ,3-hydroxy butyric acid (3-HB) ,lactic acid ,acetate ,N-acetyl glycoprotein (NAc) ,acetone ,acetoacetate ,succinate ,polyunsaturated fatty acids (PUFA) ,creatine ,glycerophosphocholine (GPC) were down-regulated;while low density lipoprotein (LDL) ,LDL/very low den-sity lipoprotein(LDL/VLDL)and glucose obviously up-regulated .Of the 14 myocardial differential metabolites ,amino acids (ala-nine ,lysine ,glutamate ,glutamine ,aspartate ,taurine ,glycine ,threonine) ,GPC ,creatine ,lactic acid ,adenosine monophos-phate (AMP) ,nicotinamide adenine dinucleotide (NAD+ ) were significantly decreased ,and glucose was up-regulated .Following EA treatment ,most of the decreased serum differential metabolites except acetone ,acetoacetate and PUFA ,and the increased serum LDL ,LDL/VLDL and glucose recovered ,basically close to the control level ;and the decreased myocardial creatine ,GPC and NAD+ were also apparently up-regulated and the increased myocardial glucose was down-regulated .But ,myocardial threo-nine and AMP still presented a decreasing state .Although the pattern of myocardial differential metabolites of the EA group had a trend to be close to the control group ,the significant difference still existed ,while the metabolic pattern of serum metabolites in the EA group was close to that of the control group .Conclusion EA stimulation of PC 6 can regulate serum or/and myocardial metabolites as amino acids ,carbohydrates ,lipids ,etc .in MIRI rats ,of which both serum and myocardial creatine ,GPC and glu-cose may be jointly confer a favorable potential for EA-induced improvement of MIRI .关键词
电针/心肌缺血再灌注损伤/特征代谢物/血清/左心室肌/代谢组学/核磁共振Key words
Electroacupuncture/Myocardial ischemia reperfusion injury/Differential metabolites/Serum/Left ventricle myocardium/Metabonomics/Nuclear magnetic resonance分类
医药卫生引用本文复制引用
唐雅妮,陈美琳,李姣兰,朱鼎铭,谭成富,刘薇薇,严洁,王超,刘密,林东海,黄彩华,杜琳..基于核磁共振氢谱技术研究电针"内关"穴对心肌缺血再灌注损伤大鼠血清及心肌组织代谢物的影响[J].针刺研究,2018,43(3):152-162,11.基金项目
国家自然科学基金面上项目(No.81574080) （No.81574080）
湖南省中医药管理局课题(No.201521) （No.201521）
湖南省高校创新平台开放基金项目(No.15k093) （No.15k093）
