针刺研究2018，Vol.43Issue(3)：163-168,6.DOI:10.13702/j.1000-0607.170705
电针对睾酮低下老年大鼠睾丸间质细胞慢性炎性反应的影响
Electroacupuncture Intervention Improves Physical Power of Aged Rats with Low Testosterone by Reducing Chronic Inflammatory Response of Leydig Cells
摘要
Abstract
Objective To assess the effect of electroacupuncture (EA) on chronic inflammatory response of Leydig cells in aged rats with low testosterone ,so as to investigate its underlying mechanism of anti-male reproductive aging .Methods Twenty-four 20 months old SD rats were randomly divided into EA ,medication and aged control groups ( n= 8 in each) ,and other 8 young SD rats (2 months of age) were used as the youth control group .EA (2 Hz/100 Hz ,1 mA) was applied to"Guanyuan"(CV 4) and bilate-ral"Shenshu"(BL 23) for 15 min ,once daily for 8 weeks except the weekends .The medication group received abdominal subcu-taneous injection of testosterone propionate (7 mg · kg-1 · 3 d-1 ) for 8 weeks .The aged control group and the youth control group received subcutaneous injection of 0 .9％ normal saline ,with the same dose and same treatment frequency as those of the medication group .The rats' physical power was assessed according to the exhausted swimming duration ,and the levels of serum total testosterone (TT) and free testosterone(FT) were determined by ELISA .The pathological changes of the testis tissue were detected by using H .E .staining ,and the immunoactivity of cyclooxygenase-2 (COX-2) in Leydig cel s was detected by immunohisto-chemistry .The expression levels of nuclear factor-κB p 65 (NF-κB p 65) ,COX-2 ,interleukin-1 β (IL-1 β) and tumor necrosis factor-α (TNF-α) proteins in the testis tissues were determined by Western blot .Results Before and after treatment ,the exhaustive swimming duration and the levels of serum TT and FT in the aged control group were significantly lower than those of the youth control group ( P < 0 .01) .After the treatment ,the exhaustive swimming duration and serum TT and FT in the EA and medi-cation groups were notably higher than those in the aged control group ( P<0 .01) .HE staining showed that the incompleteness of basement membrane of spermatogenic tubules ,reduction of spermatogenic cells and supporting cells and irregularity of Leydig cells in the testis tissue of the aged rats were relatively milder after EA intervention .Compared with the youth control group ,the expression levels of NF-κB p 65 and COX-2 ,IL-1β and TNF-αin the testicular tissue were significantly higher in the aged control group ( P<0 .01) ,while in compared with the aged control group ,the expression levels of NF-κB p 65 ,COX-2 ,IL-1β and TNF-αproteins were significantly down-regulated in the EA group ( P<0 .01) .Conclusion EA intervention can improve the physical power of the aged rats with low testosterone ,which may be related to its effects in up-regulating TT and FT levels ,and in reducing chronic in-flammatory response in the testis tissue .关键词
电针/生殖衰老/睾丸间质细胞/慢性炎性反应/力竭游泳Key words
Electroacupuncture/Reproductive aging/Leydig cell of testis/Chronic inflammation/Exhausted swimming分类
医药卫生引用本文复制引用
姚太万,刘嘉,李学智,马琳,吴家鹏,席小芳,傅燕..电针对睾酮低下老年大鼠睾丸间质细胞慢性炎性反应的影响[J].针刺研究,2018,43(3):163-168,6.基金项目
国家自然科学基金项目(No.81303036) （No.81303036）
重庆市基础科学与前沿技术研究项目(No.cstc2017jcyjAX0213) （No.cstc2017jcyjAX0213）
重庆市渝中区科技计划项目(No.20160108) （No.20160108）
