针刺研究2018，Vol.43Issue(3)：169-174,6.DOI:10.13702/j.1000-0607.170299
不同刺激强度电针对糖尿病胃轻瘫大鼠胃肠运动功能及胃窦平滑肌Ras同源物基因组成员A/相关卷曲螺旋蛋白激酶信号表达的影响
Effect of Different Stimulating Strength of Electroacupuncture on Gastrointestinal Motility and RhoA/ROCK Signaling in Gastric Antral Smooth Muscle in Diabetic Gastroparesis Rats
摘要
Abstract
Objective To observe the effect of different strength of electroacupuncture (EA) stimulation on gastrointesti-nal motility and Ras homolog gene family member (RhoA)/Rho associated coiled-coil forming protein kinase (ROCK) signaling in diabetic gastroparesis (DGP) rats ,so as to reveal the underlying mechanisms of EA for improving DGP .Methods Sixty SD rats were randomly and equally divided into blank control ,DGP model ,weak EA ,medium EA ,and strong EA groups (n=12 rats in each) .The DGP model was established by intraperitoneal injection of streptozotocin (STZ ,55 mmol/kg ,2％ ) and high-sugar and high-fat fodder feeding for 8 weeks .EA (0 .12 ,0 .24 ,0 .36 mA ,20 Hz/100 Hz) was applied to"Zusanli" (ST 36) ,"Sanyinjiao"(SP 6) and"Liangmen" (ST 21) for 20 min ,once daily for 15 successive days .Blood glucose levels were measured weekly with blood glucose meter and blood glucose test paper .Fecal phenol red excretion method was used to display gastric emptying and small intestinal propulsion function .The expression of RhoA protein in the gastric antral smooth muscle tissue was detected by immunohistochemistry and Western blot (WB) ,separately ,and that of ROCK ,myosin phosphatase target subunit 1 (MYPT 1) and phosphorylated (p)-MYPT 1 proteins in gastric antrum detected by WB .Results Compared with the blank control group ,the gastric emptying rate and smal intestine propulsion rate of the model group were significantly decreased ( P<0 .05) ,and the blood glucose level was remarkably increased ( P<0 .05) .Moreover ,the expression levels of RhoA ,ROCK ,MYPT 1 and p-MYPT 1 proteins in the gas-tric antrum were significantly down-regulated relevant to the control group ( P<0 .05) .After administration of EA ,the decreased gastric emptying rate and intestinal propulsion rate ,and the down-regulated expression of RhoA ,ROCK ,MYPT 1 and p-MYPT 1 proteins were significantly increased in the strong ,medium and weak EA stimulation groups ( P<0 .05) .Comparison among the 3 EA groups showed that the strong stimulation was significantly superior to weak stimulation in up-regulating the expression of RhoA ,ROCK ,MYPT 1 and p-MYPT 1 proteins ,and obviously superior to the medium stimulation in up-regulating RhoA and MYPT 1 protein levels ( P<0 .05) ,while the medium stimulation was significantly stronger than the weak stimulation in up-regulating the expression of ROCK ,MYPT 1 and p-MYPT 1 proteins ( P<0 .05) .There were no significant differences among the 3 EA groups in up-regulating the gastric emptying rate and small intestinal propulsion rate ,and between the strong stimulation and medium stimulation in the expression levels of ROCK and p-MYPT 1 proteins ( P>0 .05) .Conclusion Electroacupuncture stimulation of ST 36-SP 6-ST 21 at 0 .12 ,0 .24 and 0 .36 mA can promote the gastrointestinal motility in DGP rats ,which may be associated with its effects in enhancing RhoA/ROCK signaling in the gastric antral smooth muscle at different degrees .关键词
糖尿病胃轻瘫/电针/刺激量/胃肠运动/RhoA/ROCK信号/胃窦平滑肌Key words
Diabetic gastroparesis/Electroacupuncture/Stimulating strength/Gastrointestinal motility/RhoA/ROCK sig-naling/Gastric antral smooth muscle分类
医药卫生引用本文复制引用
吴雪芬,陈小丽,郑雪娜,郭鑫,谢志强,刘丽,魏歆然,岳增辉..不同刺激强度电针对糖尿病胃轻瘫大鼠胃肠运动功能及胃窦平滑肌Ras同源物基因组成员A/相关卷曲螺旋蛋白激酶信号表达的影响[J].针刺研究,2018,43(3):169-174,6.基金项目
国家重点基础研究发展计划"973"项目(No.2014CB543102) （No.2014CB543102）
国家自然科学基金项目(No.81673886) （No.81673886）
