针刺研究2018，Vol.43Issue(3)：175-179,5.DOI:10.13702/j.1000-0607.170121
电针心经与肺经对急性心肌缺血模型大鼠心肌组织超极化激活的环核苷酸门控通道2表达的影响
Effect of Electroacupuncture Stimulation of Heart and Lung Meridians on Expression of Myocar-dial HCN 2 in Acute Myocardial Ischemia Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) stimulation of Heart and Lung meridians on the expression of hyperpolarization-activated cyclic nucleotide gated potassium channel 2 (HCN 2) mRNA and protein in the myocar-dium of the left ventricle in acute myocardial ischemia (AMI) rats ,so as to underlying its mechanism in improving ischemic myo-cardial injury .Methods A total of 120 male SD rats were randomly divided into normal control ,model ,EA-Heart Meridian (HT)andEA-LungMeridian(LU)groups(n=30ratsineachgroup).TheAMImodelwasestablishedbyocclusionoftheanterior descending branch of coronary artery .EA (1 mA ,10 Hz) was applied to bilateral "Shenmen"(HT 7) or"Taiyuan"(LU 9) and the spot about 1 mm apart from the HT 7 or LU 9 on the axopetal end for 15 min on the second day after modeling ,once a day for 7 days .The left ventricular myocardium tissue was sampled for determining the expression levels of HCN 2 mRNA and protein with qPCR and Western blot ,separately .Results After AMI ,both HCN 2 mRNA and protein expression levels in the model group were significantly decreased in comparison with the normal control group ( P<0 .01) .Following EA intervention ,the expression levels of HCN 2 mRNA and protein expressions were significantly up-regulated relevant to the model group ( P<0 .01) .The effects were more obvious in the EA-HT group than in the EA-LU group ,and the expression levels were higher in the left ventricular myocardium tissue sampled on the next day after the treatment than those in the tissue sampled immediately after the treatment ( P<0 .01) .Conclusion Both EA stimulation of Heart Meridian and Lung Meridian can improve the expression of HCN 2 mRNA and protein in the myocardium in AMI rats ,which existing a delayed effect and meridian specificity .关键词
心肌缺血/电针/心经/肺经/超极化激活的环核苷酸门控通道2Key words
Myocardial ischemia/Electroacupuncture/Heart Meridian/Lung Meridian/Hyperpolarization-activated cyclic nucleotide gated potassium channel 2分类
医药卫生引用本文复制引用
吴子建,王洁,段文秀,龚长平,胡玲..电针心经与肺经对急性心肌缺血模型大鼠心肌组织超极化激活的环核苷酸门控通道2表达的影响[J].针刺研究,2018,43(3):175-179,5.基金项目
国家自然科学基金面上项目(No.81574083、81473783) （No.81574083、81473783）
安徽高校自然科学研究重点项目(No.KJ2017A300) （No.KJ2017A300）
安徽高校科研创新平台建设项目(No.2015TD033) （No.2015TD033）
