中国病理生理杂志2018，Vol.34Issue(3)：385-392,8.DOI:10.3969/j.issn.1000-4718.2018.03.001
室旁核内脂肪酸酰胺水解酶上调介导慢性心衰大鼠交感神经兴奋亢进
Upregulated FAAH expression in PVN contributes to sympathoexcitation in rat with chronic heart failure
摘要
Abstract
AIM:To investigate the expression of fatty-acid amide hydrolase(FAAH)in paraventricular nu-cleus(PVN)and its contribution to renal sympathetic nerve activity in rats with chronic heart failure(CHF).METH-ODS:A rat model of CHF was established by ligation of the left coronary artery to induce acute myocardial infarction. Eight weeks after ischemia,the rat model of CHF was identified by echocardiogram and histopathological observation.The plasma level of norepinephrine(NE)was detected by ELISA.The protein expression levels of FAAH in the PVN were de-termined by Western blot.The N-arachidonoylethanolamide(AEA)generation in PVN was analyzed by high-performance liquid chromatography.After microinjection of AEA,PF3845(an FAAH inhibitor)or rAAV2-FAAH shRNA virus in PVN, the sympathetic drive indexes were recorded in different experiment groups.RESULTS: Compared with the rats in sham group,the cardiac function and AEA concentration in PVN were significantly reduced, while the plasma NE level and FAAH expression in PVN were obviously increased in the CHF rats(P<0.05).After microinjecion of PF3845, AEA or rAAV2-FAAH shRNA virus in PVN, the sympathetic drive indexes were decreased significantly and the cardiac function were improved in the CHF rats.CONCLUSION:Upregulated FAAH expression in PVN may result in sympathoexcitation in the rat with CHF.关键词
室旁核/慢性心力衰竭/交感神经兴奋/脂肪酸酰胺水解酶/N-花生四烯酰乙醇胺Key words
Paraventricular nucleus/Chronic heart failure/Sympathoexcitation/Fatty-acid amide hydrolase/N-arachidonoylethandamide分类
医药卫生引用本文复制引用
王仁俊,周琴,未晓巍,李华,赵永斌,齐云峰,栾剑,周晓馥..室旁核内脂肪酸酰胺水解酶上调介导慢性心衰大鼠交感神经兴奋亢进[J].中国病理生理杂志,2018,34(3):385-392,8.基金项目
国家自然科学基金资助项目(No.81202527) （No.81202527）
吉林省科技发展计划项目(No.20130522002JH) （No.20130522002JH）
吉林省教育厅"十二五"科学技术研究项目(No.2015212) （No.2015212）
