中国病理生理杂志2018，Vol.34Issue(3)：410-416,7.DOI:10.3969/j.issn.1000-4718.2018.03.005
PRRX2通过调控Wnt/β-catenin信号通路促进胃癌细胞活力和迁移
Promoting cell viability and migration of gastric cancer cells by PRRX2 via activation of Wnt/β-catenin signaling pathway
摘要
Abstract
AIM:To study the effect of paired-related homeobox 2(PRRX2)gene on the viability and migra-tion ability of gastric cancer cells,and to analyze the underlying mechanism of regulating Wnt /β-catenin signaling pathway. METHODS:The expression of PRRX2 in gastric cancer and normal gastric tissue and the correlation between PRRX 2 ex-pression in gastric cancer tissues with the overall survival rate of gastric cancer patients were analyzed by bioinformatics. The small interfering RNA(siRNA)and over-expressed plasmids of PRRX2 were transfected into gastric cancer cells MGC-803 and SGC-7901,respectively.MTT assay and Transwell assay were used to detect the viability and migration ability of gastric cancer cells.Western blot and TOPflash/FOPflash dual-luciferase reporter gene assay were used to detect the activi-ty of Wnt/β-catenin signaling pathway.Co-immunoprecipitation was used to detected the interaction between PRRX 2 and β-catenin proteins.RESULTS:Knockdown of PRRX2 attenuated the viability and migration ability of gastric cancer cell line MGC-803(P <0.05).Over-expression of PRRX2 enhanced the viability and migration ability of SGC-7901 cells (P<0.05),increased the protein levels of β-catenin, c-Myc and cyclin D1(P<0.05)and the activity of TOPflash/FOPflash dual-luciferase reporter gene(P<0.05).PRRX2 interacted with β-catenin protein in gastric cancer cells.CON-CLUSION:PRRX2 promotes the viability and migration ability of gastric cancer cells, which may be related to Wnt/β-catenin signaling pathway.关键词
胃癌/配对相关同源框2/Wnt/β-catenin信号通路/细胞活力/细胞迁移Key words
Gastric cancer/Paired-related homeobox 2/Wnt/β-catenin signaling pathway/Cell viability/Cell migration分类
医药卫生引用本文复制引用
王倩,陈冬玲,张瓅方,卞华..PRRX2通过调控Wnt/β-catenin信号通路促进胃癌细胞活力和迁移[J].中国病理生理杂志,2018,34(3):410-416,7.基金项目
国家自然科学基金资助项目(No.81373627) （No.81373627）
河南省张仲景方药与免疫调节重点实验室开放课题资助项目(No.kfkt201703) （No.kfkt201703）
