中国病理生理杂志2018，Vol.34Issue(3)：417-422,6.DOI:10.3969/j.issn.1000-4718.2018.03.006
黄芩素抑制胃癌MGC-803细胞增殖和迁移
Baicalein inhibits proliferation and migration of gastric cancer MGC-803 cells
摘要
Abstract
AIM: To investigate the effects of baicalein(BAI)on the proliferation and migration of gastric cancer MGC-803 cells and the mechanisms.METHODS:After MGC-803 cells were treated with BAI at different concen-trations,the viability of the MGC-803 cells was tested by MTT assay.The cell colony formation ability were detected by plate colony formation assay.Wound-healing and Transwell cell migration assays were used to test the migration ability of the MGC-803 cells.The concentration of 12-hydroxyeicosatetraenoic acid(12-HETE)was measured by ELISA.The pro-tein levels of platelet type 12-lipoxygenase(p12-LOX),vascular endothelial growth factor(VEGF),p-ezrin and epithelial-mesenchymal transition(EMT)markers in MGC-803 cells were determined by Western blot.RESULTS:BAI significantly inhibited the proliferation,plate colony formation and migration abilities of the MGC-803 cells(P<0.05 or P<0.01), down-regulated the concentration of p12-LOX metabolite 12-HETE significantly(P<0.05 or P<0.01), decreased the protein levels of p12-LOX,VEGF,p-ezrin,vimentin and Snail(P<0.05 or P<0.01),and increased the protein expres-sion of E-cadherin(P<0.01).CONCLUSION:BAI suppresses the proliferation and migration abilities of gastric cancer MGC-803 cells effectively.These effects of BAI may be related to regulating the protein levels of p12-LOX,VEGF,p-ezrin and EMT-related proteins.关键词
黄芩素/胃癌/细胞增殖/细胞迁移Key words
Baicalein/Gastric cancer/Cell proliferation/Cell migration分类
医药卫生引用本文复制引用
孙沛林,朴日龙,王莹,任香善,陈丽艳,林贞花,朴英实..黄芩素抑制胃癌MGC-803细胞增殖和迁移[J].中国病理生理杂志,2018,34(3):417-422,6.基金项目
国家自然科学基金资助项目(No.31460294) （No.31460294）
吉林省教育厅“十三五”科学技术研究项目(吉教科合字[2016]第263号 （）
