中国病理生理杂志2018，Vol.34Issue(3)：458-463,6.DOI:10.3969/j.issn.1000-4718.2018.03.013
miR-195对肺癌A549细胞生物学行为的调控作用
Regulatory effects of miR-195 on biological behaviors of lung cancer A549 cells
王依 1詹蓉 1高建生 1张冬英 1刘华1
作者信息
- 1. 广东药科大学附属第一医院干保科,广东广州510080
- 折叠
摘要
Abstract
AIM: To investigate the regulatory effects of microRNA(miR)-195 on the biological behaviors, such as viability,apoptosis and migration, of lung cancer A549 cells, and to explore the related mechanisms.METH-ODS:After miR-195 mimics were transfected into the A549 cells,the cell viability, cell cycle distribution and apoptosis were measured by CCK-8 assay and flow cytometry.Transwell assay was used to detect cell migration ability.Furthermore, the protein levels of cyclin D1,CDK2,Bcl-2 and p-Rb/Rb were determined by Western blot.Dual-luciferase reporter as-say was used to screen and identify the possible target genes of miR-195.RESULTS: Over-expression of miR-195 in the A549 cells inhibited the cell viability and induced cell cycle arrest,accompanied with the decrease in the cell migration a-bility and the increase in the apoptotic rate(P<0.05).Furthermore,the protein levels of cyclin D1,CDK2,Bcl-2 and p-Rb were significantly decreased(P<0.05).Dual-luciferase reporter assay demonstrated that MYB was a potential target gene of miR-195.Over-expression of MYB in the A549 cells partially reversed the effects of miR-195 on the cell viability, apoptosis and migration.CONCLUSION: miR-195 inhibits lung cancer A549 cell growth and migration, and promotes cell apoptosis by targeting MYB gene.关键词
微小RNA-195/肺癌/细胞活力/细胞凋亡/细胞迁移/MYB基因Key words
MicroRNA-195/Lung cancer/Cell proliferation/Apoptosis/Cell migration/MYB gene分类
医药卫生引用本文复制引用
王依,詹蓉,高建生,张冬英,刘华..miR-195对肺癌A549细胞生物学行为的调控作用[J].中国病理生理杂志,2018,34(3):458-463,6.
