中国感染控制杂志2018，Vol.17Issue(4)：294-298,5.DOI:10.3969/j.issn.1671-9638.2018.04.004
广州市ICU环境中耐碳青霉烯类鲍曼不动杆菌的克隆相关性
Clone correlation of carbapene m-resistant Acinetobacter baumannii in en-vironment of intensive care units in Guangzhou City
摘要
Abstract
Objective To understand the clone correlation of carbapenem-resistant Acinetobacter baumannii (CRAB)in the environment of intensive care units(ICUs)in Guangzhou City,identify genotypes,and provide basis for prevention and control of healthcare-associated infection(HAI).Methods 39 strains of CRAB isolated from en-vironment of ICUs in 7 hospitals in Guangzhou City were collected,susceptibility to 10 kinds of antimicrobial agents was detected by Kirby-Bauer method,OXA gene of strains was detected by polymerase chain reaction(PCR),clone polymorphism analysis was performed with pulsed-field gel electrophoresis(PFGE)and multilocus sequence typing (MLST).Results Among 39 strains of CRAB,resistance rate to levofloxacin was the lowest(56.4%),resistance rates to other 9 antimicrobial agents were all>90%. PCR results showed that 39 strains(100%)of CRAB all car-ried OXA-51 gene,37(94.9%)carried OXA-23 gene,but OXA-24 and OXA-58 genes were not found. PFGE showed that 38 CRAB strains were divided into 5 clones,group A was the main epidemic clone,MLST analysis showed that the main clone of CRAB was ST195.Conclusion Transmission of CRAB clone carrying OXA-23 gene exists in the ICU environment of Guangzhou City,cleaning and disinfection of ICU environment should be intensi-fied,so as to reduce HAI caused by CRAB.关键词
鲍曼不动杆菌/克隆相关性/碳青霉烯酶基因/脉冲场凝胶电泳/多位点序列分型Key words
Acinetobacter baumannii/clone correlation/carbapenemase gene/pulsed-field gel electrophoresis/multilocus sequence typing分类
医药卫生引用本文复制引用
林云万,周勇,张旭,李晓宁,赵正阳,刘远..广州市ICU环境中耐碳青霉烯类鲍曼不动杆菌的克隆相关性[J].中国感染控制杂志,2018,17(4):294-298,5.基金项目
广东省医学科研基金项目(A2015426)；广东省医学科研基金项目(B2016007) （A2015426）
