中国骨伤2017，Vol.30Issue(12)：1113-1117,5.DOI:10.3969/j.issn.1003-0034.2017.12.008
50Hz 1.8mT正弦交变电磁场对生长期大鼠骨密度的影响研究
Effect of 50 Hz 1.8 mT sinusoidal electromagnetic fields on bone mineral density in growing rats
摘要
Abstract
Objective:To study effects of 50 Hz 1.8 mT sinusoidal electromagnetic fields (SEMFs) on bone mineral density (BMD) in SD rats.Methods:Thirty SD rats weighted (110±10) and aged 1 month were randomly divided into control group and electromagnetic field group,15 in each group.Normal control group of 50 Hz 0 mT density and sinusoidal electromagnetic field group of 50 Hz 1.8 mT were performed respectively with 1.5 h/d and weighted weight once a week,and observed food-intake.Rats were anesthesia by intraperitoneal injection and dual energy X-ray absorptiometry were used to detect bone density of whole body,and detected bone density of femur and vertebral body.Osteocalcin and tartrate-resistant acid phos phatase 5b were detected by ELSA;weighted liver,kidney and uterus to calculate purtenance index,then detected pathologic results by HE.Results:Compared with control group,there was no significant change in weight every week,food-intake every day;no obvious change of bone density of whole body at 2 and 4 weeks,however bone density of whole body,bone density of excised femur and vertebra were increased at 6 weeks.Expression of OC was increased,and TRACP 5b expression was decreased.No change of HE has been observed in liver,kidney and uterus and organic index.Conclusion:50 Hz 1.8 mT sinu soidal electromagnetic fields could improve bone formation to decrease relevant factors of bone absorbs,to improve peak bone density of young rats,in further provide a basis for clinical research electromagnetic fields preventing osteoporosis foundation.关键词
电磁场/骨密度/大鼠Key words
Electromagnetic fields/Bone density/Rats引用本文复制引用
高玉海,周延峰,李少锋,李文苑,葸慧荣,杨芳芳,陈克明..50Hz 1.8mT正弦交变电磁场对生长期大鼠骨密度的影响研究[J].中国骨伤,2017,30(12):1113-1117,5.基金项目
国家自然科学基金(编号:81270963) （编号:81270963）
国家国际科技合作专项项目(编号:2015DFR30940) （编号:2015DFR30940）
甘肃省空间辐射生物学重点实验室开放基金(编号:2016GKL002)Supported by National Natural Science Foundation of China (No.81270963) （编号:2016GKL002）
