中国免疫学杂志2018，Vol.34Issue(3)：349-353,5.DOI:10.3969/j.issn.1000-484X.2018.03.007
脯氨酰异构酶1沉默抑制缺氧/复氧H9c2心肌细胞凋亡
Inhibitory effects of Pin1 silencing by siRNA on apoptosis of H9c2 cardiomyo-cytes induced by hypoxia/reoxygenation
摘要
Abstract
Objective:Pin1 plays an important role in the pathogenesis of cardiovascular disease,our study aims to investigate the effects of Pin1 silencing by siRNA on H9c2 apoptosis induced by hypoxia/reoxygenation.Methods:H9c2 cells were cultured and subjected to a hypoxia/reoxygenation (H/R) condition in vitro,mimicking ischemic/reperfusion injury in vivo.The mRNA and protein expression of Pin1 were detected by RT-qPCR and Western blot.H9c2 cells were divided into control group,H/R group,H/R+Pin1 siRNA group,H/R+scramble siRNA group.MTT and flow cytometry with Annexin V-FITC/PI staining were respectively performed to detect cell viability and apoptosis.The expression of Bax and Bcl-2 were measured by Western blot.The activity of Caspase-3 was detected by automatic biochemistry analytic instrument.Results:The mRNA and protein levels of Pin1 were highly expressed in the cells of H/R group.Transfection with Pin1 siRNA strikingly inhibited the expression of Pin1.Compared with H/R group,Pin1 siRNA markedly increased cell viability,decreased the cell apoptosis and the Caspase-3 activity.Furthermore,the increased Bcl-2,decreased Bax and the ratio of Bcl-2 to Bax were observed in Pin1 siRNA group (P<0.05) compared with H/R group.Conclusion:Downregulation of Pin1 protects hypoxia/reoxygenation-injured H9c2 cells from apoptosis,which is possibly through the upregulation of Bcl-2 and downregulation of Bax and Caspase-3 activity.关键词
脯氨酰异构酶1/H9c2细胞/缺氧/复氧/凋亡Key words
Pin1/H9c2 cells/Hypoxia/reoxygenation/Apoptosis分类
医药卫生引用本文复制引用
郭红雨,段永珂,何瑞利,程冠昌..脯氨酰异构酶1沉默抑制缺氧/复氧H9c2心肌细胞凋亡[J].中国免疫学杂志,2018,34(3):349-353,5.基金项目
本文为河南省科技厅医学科技攻关基金资助项目(112102310137). （112102310137）
