中国农业大学学报2018，Vol.23Issue(4)：8-13,6.DOI:10.11841/j.issn.1007-4333.2018.04.02
磁小体的碱性磷酸酶活性分析
Analysis of alkaline phosphatase activity of magnetosome
摘要
Abstract
An experiment was carried out to determine alkaline phosphatase-like activity in magnetosomes and whether the magnetosomes could act as the solid carrier of magnetic immune ELISA.The activity of the magnetosome and the commercial alkaline phosphatase was compared under different coloring time,temperature and pH.The results showed that the time used for the color development of commercial alkaline phosphatase was very short and sensitive.However,the temperature and pH had certain limitations.The trace of the magnetosome required long time in color development,temperature and pH had little effect on the color reaction of magneto`some.Moreover,the location of the magnetophilic alkaline phosphatase on magnetosome was preliminarily determined by the treatment of different protein denaturants.Based on these findings,it was concluded that the weak chromogenic ability of the magnetosome was not significantly affected when it was used for magnetic immunoassay ELISA.Therefore,the magneto`some could be used as a solid carrier for magnetic immune ELISA.关键词
磁小体/固相载体/碱性磷酸酶/磁性免疫ELISA/趋磁细菌Key words
magnetosome/solid phase carrier/alkaline phosphatase/magnetic immune ELISA/magnetotactic bacteria分类
生物科学引用本文复制引用
徐俊杰,王曼曼,刘凌子,李峰,田杰生..磁小体的碱性磷酸酶活性分析[J].中国农业大学学报,2018,23(4):8-13,6.基金项目
中国国家自然科学基金Grant No.31570037 （）
农业生物技术国家重点实验室开放研究重点课题Grant No.2015SKLAB6-2 （）
