中国农业大学学报2018，Vol.23Issue(4)：34-44,11.DOI:10.11841/j.issn.1007-4333.2018.04.05
根癌农杆菌介导的香石竹遗传转化体系建立
Agrobacterium-mediated genetic transformation system of carnation
摘要
Abstract
In order to optimize transformation system and obtain stable transgenic plants,the effects of Agrobacterium tumefaciens strains,carnation genotype,the number of pre-culture days,the way of infection,the mode and time of coculture on the leaf genetic transformation of carnation were studied.The optimized carnation transformation system was as follows:Shaking with Agrobacterium tumefaciens buffer for 12 min (200 r/min) and standing for 3 min after 2 d preculture;Then co-culture for 5 d on three-layer sterile filter paper soaked with 50 mol/L AS solution.The results of different genotype comparion showed that the differentiation rate of resistant buds of ‘Mallow Titanium Alloy'was higher than ‘Master'and ‘Pink Shirt'.Treatment of 60 mg/L Kan had good select effect for all carnation genotypes.Medium of 0.5 mg/L IBA + 0.22 mg/L TDZ was direct germination medium with high differentiation efficiency,high budding rate and good growth status.Optimum genetic transformation system of carnation was established with high reproducibility,high transformation efficiency and low genotype dependence in this study.DcaPIP1;1 gene transformation efficiency was improved by using this system.关键词
香石竹/农杆菌介导/遗传转化体系/高效Key words
carnation/agrobacterium-mediated/genetic transformation system/high efficiency分类
生物科学引用本文复制引用
孔维龙,王翠,但乃震,李丹丹,包满珠,傅小鹏..根癌农杆菌介导的香石竹遗传转化体系建立[J].中国农业大学学报,2018,23(4):34-44,11.基金项目
国家自然科学基金项目(31000918) （31000918）
中央高校基本科研业务费专项(2662015PY052,2662016PY041) （2662015PY052,2662016PY041）
