中国农业大学学报2018，Vol.23Issue(4)：45-52,8.DOI:10.11841/j.issn.1007-4333.2018.04.06
猪源嗜麦芽寡养单胞菌的分离鉴定与生物学分析
Isolation, identification and biological characteristics analysis of Stenotrophomonas maltophila
摘要
Abstract
The aim of this study was to investigate the biological characteristics and provide references for Stenotrophomonas maltophila identification,diagnosis and treatment.The dyeing characteristics and culture characteristics of the isolate was observed and identified by biochemical tests.To determine LD50 of the isolate in mice,drug sensitive test was conducted to analyze the toxicity and drug resistance.A system evolutionary tree was built based on the result of 16S rRNA gene sequence analysis.The results of the 16S rRNA sequencing of the isolated strain showed 99％ homology with S.maltophila.The colonies of the isolated strain on TSA agar appeared to be smooth,circular and translucent and had α-hemolysis on blood agar.The LD50 of isolated strain to mice was 6.62 × 107 cfu per mouse.The results of antibiotic sensitivity experiment showed that it was sensitive to cephalosporin antibiotics but insensitive to quinolone,tetracycline,cephalosporin antibiotics,carbapenems and aminoglycoside antibiotics.In conclusion,this pathogen was identified to be S.maltophila.In addition,it was proved to have strong pathogenicity and powerful drug resistance.This study provided references for the clinical diagnosis,disease prevention and drug treatment of associate streptococcus disease.关键词
嗜麦芽寡养单胞菌/16S rRNA/分离鉴定/人兽共患病Key words
Stenotrophomonas maltophila/16S rRNA/isolation and identification/zoonosis分类
农业科技引用本文复制引用
张鹏飞,王印,杨泽晓,姚学萍,张博..猪源嗜麦芽寡养单胞菌的分离鉴定与生物学分析[J].中国农业大学学报,2018,23(4):45-52,8.基金项目
“十二五”国家科技支撑计划项目(2013BAD12B04) （2013BAD12B04）
