中国全科医学2018，Vol.21Issue(5)：546-550,5.DOI:10.3969/j.issn.1007-9572.2017.00.099
柯萨奇病毒A组16型病毒株VP1基因序列测定及进化分析研究
VP1 Sequence Determination and Phylogenetic Analysis of Coxsackievirus A16 Strains
摘要
Abstract
Objective To determine the VP1 sequence of coxsackievirus A16(CA16) strains from Hangzhou, China in 2015, and based on this, to investigate the prevalence and phyletic evolution of CA16 in this district,in order to provide a reference for the prevention and treatment of hand, foot and mouth disease(HFMD) and the selection of prophylactic vaccine against HFMD related to CA16. Methods A total of 256 children who were diagnosed as HFMD and received treatment in Hangzhou Children's Hospital in 2015 and had complete clinical data were enrolled.Their culture test results of throat swabs or stool samples were collected.By RT-PCR, VP1 sequencewas amplified (about 1 020 bp).DNASTAR and Primer Premier 5.0 were used for comparative analysis of the amplified products and the complete CA16 sequences in the NCBI database. Mega 3.1 software was used to establish VP1 sequences and GenBank CA16 reference strain VP1 sequence phylogenetic tree.Results Totaled 80 strains of CA16 virus were isolated. Compared with the CA16 sequences in the NCBI database, the nucleotide homology, amino acid homology for CA16 sequences from Hangzhou were 90.8%-100.0%, 99.5%-100.0%, respectively. Phylogenetic tree analysis showed that, all the 17 CA16 strains belong to B1 subtype, including 2 evolutionary branches, B1a(4 strains) and B1b(13 strains). Conclusion The CA16 strains from Hangzhou in 2015 belong to B1 subtype, which is consistent with the results of molecular evolution and pathogenesis of HFMD proposed in some domestic and abroad researches.Therefore, this kind of CA16 strainsis were showed promise for further study as a vaccine candidate strain against HFMD.关键词
手足口病/柯萨奇病毒感染/柯萨奇病毒A组16型/VP1/系统进化分析Key words
Hand/foot and mouth disease/Coxsackievirus infections/Coxsackievirus A16/VP1/Phylogenetic analysis分类
医药卫生引用本文复制引用
李小平,吴亦栋,周俊,周林福,樊凯,钱苗苗..柯萨奇病毒A组16型病毒株VP1基因序列测定及进化分析研究[J].中国全科医学,2018,21(5):546-550,5.基金项目
2015年浙江省教育厅科研资助项目(Y201533677) （Y201533677）
