中国人兽共患病学报2018，Vol.34Issue(1)：29-34,38,7.DOI:10.3969/j.issn.1002-2694.2018.00.001
乙型肝炎病毒剪接蛋白HBSP与泛转录表达因子剪接变异体1相互作用增强NF-κB活性
Hepatitis B spliced protein enhanced NF-κB activities via interaction with ubiquitously expressed transcript splice variant 1
摘要
Abstract
We investigated whether hepatitis B spliced protein affect the NF-kappa B activities by interacting with ubiquitously expressed transcript splice variant 1 (UXT-V1).The HBSP-UXT-V1 protein interactions were screened by yeast two hybrid assay,and confirmed by immunoprecipitation,confocal microscopy,mammalian two hybrid assay,and GST-Pulldown assay.The reporter plasmids driven by NF-kappa B promoter were transfected into UXT-knockdown HBSP stably expressed cell lines,and the reporter genes were detected after transfection.Results showed that the interaction between UXT-V1 and HBSP in yeast was demonstrated.Furtherly,HBSP could interact with UXT-V1 in mammalian cells.HBSP could enhance NF-kappa B activities,and this effect was partly achieved by the interaction with UXT-V1.In conclusion,the effect of HBSPUXT-V1 interaction on the NF-kappa B pathway in hepatocytes may have an impact on HBV related liver diseases.关键词
乙型肝炎病毒/RNA剪接/泛转录表达因子/NF-κBKey words
hepatitis B virus/RNA splicing/ubiquitously expressed transcript protein/nuclear factor kappa B分类
医药卫生引用本文复制引用
陈婉南,俞燕飞,吴琼,刘慎敏,吴龙飞,施佳健,林旭..乙型肝炎病毒剪接蛋白HBSP与泛转录表达因子剪接变异体1相互作用增强NF-κB活性[J].中国人兽共患病学报,2018,34(1):29-34,38,7.基金项目
Supported by the Medical Elite Cultivation Program of Fujian (No.2014-ZQN-ZD-25),the New Century Talents Supporting Plan of Fujian Education Department (No.JA13129),the Fujian Provincial Medical Innovation Project (No.2012-CX-14),and the Joint Research Program of Health and Planning Committee and Education Department of Fujian (No.WKJ-FJ-29)福建省卫生系统中青年骨干人才培养项目(No.2014-ZQN-ZD-25) （No.2014-ZQN-ZD-25）
福建省高等学校新世纪优秀人才支持计划(No.JA13129) （No.JA13129）
福建省卫生厅医学创新课题(No.2012-CX-14) （No.2012-CX-14）
福建省卫生教育联合攻关计划(No.WKJ-FJ-29)联合资助. （No.WKJ-FJ-29）
