中国水稻科学2018，Vol.32Issue(2)：103-110,8.DOI:10.16819/j.1001-7216.2018.7056
响应镉胁迫的水稻WRKY15转录因子基因的分离与表达特征
Isolation and Expression Profiles of Cadmium Stress-Responsive Rice WRKY15 Transcription Factor Gene
摘要
Abstract
[Objective]Cadmium (Cd) is one of the most toxic heavy metal elements to growth and development of plants and human health. WRKY transcription factors play important regulatory roles in the resistance against stresses. But so far, the regulatory functions of WRKY proteins (WRKYs) in the resistant responses to Cd stress in plants remain unclear so far. [Methods]Full-length coding sequence of OsWRKY15 was amplified by RT-PCR with gene-specific primers designed according to gene prediction. Structural characteristics of OsWRKY15 were analyzed by bioinformatics software;Organ-specific and induced expression patterns of OsWRKY15 were analyzed by qRT-PCR. Transcriptional activation activity of OsWRKY15 was tested by yeast one-hybrid assay.[Results]OsWRKY15, 988 bp in length, contained an entire ORF in length of 825 bp, encoding a protein of 274 amino acid residues consisting of one WRKY domain with a zinc finger motif of C2HC, belonging to the WRKY subgroup Ⅲ. OsWRKY15 had a nuclear localization signal and was predicted to be localized in nucleus. Yeast one-hybrid assay revealed that OsWRKY15 had transcriptional activation activity. The transcript levels of OsWRKY15 were higher in roots than those in stems and leaves, and the least was in panicles, flowers and dry seeds. Its expression was rapidly and significantly induced in response to Cd exposure, nitric oxide (NO) and abscisic acid (ABA) application with more increase folds in roots than in leaves. However, salicylic acid, jasmonic acid and ethylene exerted no obvious effects on the expression of OsWRKY15. [Conclusion]OsWRKY15 may be involved in the regulation of rice resistance response to Cd stress through NO- and ABA-dependent signal pathways.关键词
WRKY转录因子/镉/基因分离/基因表达模式/水稻Key words
WRKY transcription factor/cadmium/gene isolation/gene expression profiles/Oryza sativa分类
农业科技引用本文复制引用
彭喜旭,白宁宁,王海华..响应镉胁迫的水稻WRKY15转录因子基因的分离与表达特征[J].中国水稻科学,2018,32(2):103-110,8.基金项目
国家自然科学基金资助项目(31301617) （31301617）
湖南省自然科学基金资助项目(2016JJ3060) （2016JJ3060）
湖南省教育厅重点科研平台开放基金资助项目(15K045). （15K045）
